Stress-Induced Mucus Secretion and Its Composition by a Combination of Proteomics and Metabolomics of the Jellyfish Aurelia coerulea.
Stress-Induced Mucus Secretion and Its Composition by a Combination of Proteomics and Metabolomics of the Jellyfish Aurelia coerulea.
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结合蛋白质组学和代谢组学研究水母的应激诱导粘液分泌及其成分。
DOI：
10.3390/md16090341
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发表时间：
2018-09-18
期刊：
影响因子：
5.4
通讯作者：
Xiao L
中科院分区：
文献类型：
--
作者：
Liu W;Mo F;Jiang G;Liang H;Ma C;Li T;Zhang L;Xiong L;Mariottini GL;Zhang J;Xiao L
背景：水母对刺激粘液分泌作为防御的外部压力反应迅速。分泌粘液的组成和分泌过程都不清楚。方法：将奥雷利亚蓝水母从环境海水中取出，进行刺激。然后在60 min内收集分泌粘液和组织样品，并分别使用液相色谱-串联质谱（LC-MS/MS）和超高效液相色谱/四极杆飞行时间质谱（UPLC-QTOF-MS/MS）通过蛋白质组学和代谢组学的组合进行分析。结果：两个阶段的样本采集显示，体积迅速减少，然后逐渐增加。在组织匀浆和分泌粘液中分别鉴定出2421和1208种蛋白质。基因本体（GO）分析表明，粘液富集蛋白主要位于细胞外或膜相关区域，而组织富集蛋白分布在整个细胞内区室。在16种不同的代谢物中，粘液中的色胺增加了最大的倍数变化值7.8，这与其参与京都基因和基因组百科全书（KEGG）途径“色氨酸代谢”一致。我们鉴定了11种金属蛋白酶，4种丝氨酸蛋白酶抑制剂，3种超氧化物歧化酶和3种补体，推测它们的存在与自我保护防御有关。结论：我们的研究结果提供了应激诱导的粘液和组织匀浆中蛋白质和代谢产物的组成谱。天蓝色这为正在进行的发现新的生物活性化合物的努力提供了见解。粘液中色胺含量的大量增加可能表明水母在离开海水后产生了强烈的应激反应，而水母体内的酶、丝氨酸蛋白酶抑制剂和补体等活性自我保护成分可能在水母的天然免疫中起着关键作用。
Background: Jellyfish respond quickly to external stress that stimulates mucus secretion as a defense. Neither the composition of secreted mucus nor the process of secretion are well understood. Methods: Aurelia coerulea jellyfish were stimulated by removing them from environmental seawater. Secreted mucus and tissue samples were then collected within 60 min, and analyzed by a combination of proteomics and metabolomics using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) and ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UPLC-QTOF-MS/MS), respectively. Results: Two phases of sample collection displayed a quick decrease in volume, followed by a gradual increase. A total of 2421 and 1208 proteins were identified in tissue homogenate and secreted mucus, respectively. Gene Ontology (GO) analysis showed that the mucus-enriched proteins are mainly located in extracellular or membrane-associated regions, while the tissue-enriched proteins are distributed throughout intracellular compartments. Tryptamine, among 16 different metabolites, increased with the largest-fold change value of 7.8 in mucus, which is consistent with its involvement in the Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway ‘tryptophan metabolism’. We identified 11 metalloproteinases, four serpins, three superoxide dismutases and three complements, and their presence was speculated to be related to self-protective defense. Conclusions: Our results provide a composition profile of proteins and metabolites in stress-induced mucus and tissue homogenate of A. coerulea. This provides insight for the ongoing endeavors to discover novel bioactive compounds. The large increase of tryptamine in mucus may indicate a strong stress response when jellyfish were taken out of seawater and the active self-protective components such as enzymes, serpins and complements potentially play a key role in innate immunity of jellyfish.
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影响因子：
6.7
作者：
Broxton CN;Culotta VC
通讯作者：
Culotta VC
影响因子：
8.8
作者：
Hanaoka, K;Ohno, H;Irgolic, KJ
通讯作者：
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DOI：
10.1007/bf00391016
发表时间：
1994-01-01
期刊：
MOLECULAR & GENERAL GENETICS
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3.3
作者：
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通讯作者：
de Lima, Maria Elena
影响因子：
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作者：
Jatkar, Amita A.;Brown, Barbara E.;Pearson, Jeffery P.
通讯作者：
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