Energy and metabolic pathways in trefoil factor family member 2 (Tff2) KO mice beyond the protection from high-fat diet-induced obesity
Energy and metabolic pathways in trefoil factor family member 2 (Tff2) KO mice beyond the protection from high-fat diet-induced obesity
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DOI：
10.1016/j.lfs.2018.11.006
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发表时间：
2018-12-15
期刊：
影响因子：
6.1
通讯作者：
St-Amand, Jonny
中科院分区：
文献类型：
--
作者：
Ghanemi, Abdelaziz;Melouane, Aicha;St-Amand, Jonny
三叶因子家族成员2（TFF 2）是一种小肠肽。我们先前已经显示Tff 2敲除（KO）小鼠被保护免于高脂肪（HF）饮食诱导的肥胖（De Giorgio等人，2013年a）。因此，探索Tff 2 KO相关途径的小鼠在基因组，蛋白质和生化水平将使我们能够阐明这背后的过程，从obesity.Main方法的保护：为了探索与Tff 2缺陷的代谢和能量的影响，我们从以前的研究中使用的血液样本来测量血清中的游离脂肪酸，葡萄糖，甘油和甘油三酯的水平。选定的基因和蛋白质的表达水平与能量代谢的骨骼肌，肝脏和脂肪tissue.Key发现：12周后的挑战Tff 2 KO和WT小鼠HF和低脂饮食，Tff 2 KO小鼠有较低的血清葡萄糖，甘油三酯和甘油水平。重要的是，蛋白质印迹和Q_RT-PCR揭示了所选基因和蛋白质的表达水平通过氧化磷酸化增强脂质和葡萄糖的利用，从而减少脂肪储存和增加能量消耗。我们绘制了涉及脂肪组织，肝脏，骨骼肌和交感神经系统，保护Tff 2 KO小鼠免受HF饮食诱导的肥胖。我们的数据突出了Tff 2相关途径作为肥胖治疗的潜在靶点。
Aims: Trefoil factor family member 2 (TFF2) is a small gut peptide. We have previously shown that Tff2 knock out (KO) mice are protected from high-fat (HF) diet-induced obesity (De Giorgio et al., 2013a). Thus, exploring Tff2 KO-related pathways of mice at the genomic, proteinic and biochemical levels would allow us to elucidate the processes behind this protection from obesity.Main methods: To explore the metabolic and energetic effects related to Tff2 deficiency, we used sampled blood from the previous study to measure levels of free fatty acids, glucose, glycerol and triglycerides in serum. Expression levels of selected genes and proteins related to energy metabolism in the skeletal muscle, liver and adipose tissue were also studied.Key findings: Following the 12-wk challenging of Tff2 KO and WT mice with both HF and low-fat diet, Tff2 KO mice had lower levels of serum glucose, triglycerides and glycerol. Importantly, western blotting and Q_RT-PCR revealed that the expression levels of selected genes and proteins are toward less fat storage and increased energy expenditure by enhancing lipid and glucose utilization via oxidative phosphorylation.Significance: We mapped a part of the metabolic and biochemical pathways of lipids and glucose involving the adipose tissue, liver, skeletal muscle and sympathetic nervous system that protect Tff2 KO mice from the HF diet-induced obesity. Our data highlight Tff2-related pathways as potential targets for obesity therapies.