Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane.

Synthesis of proteins in cells infected with herpesvirus, VI. Characterization of the proteins of the viral membrane.
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疱疹病毒感染细胞中蛋白质的合成,VI。

DOI:
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发表时间:
1970
影响因子:
11.1
通讯作者:
T. Ben‐Porat
T. Ben‐Porat
中科院分区:
综合性期刊1区
文献类型:
--
作者:
A. Kaplan;T. Ben‐Porat

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伪狂犬病病毒粒子的包膜可以通过 Triton X-100 处理去除,而保持核衣壳完整。以这种方式去除的病毒包膜包含病毒粒子的所有糖蛋白和约 40% 的蛋白质。聚丙烯酰胺凝胶电泳分析表明,包膜蛋白迁移为四个不同的峰,所有峰都含有糖蛋白。核衣壳的蛋白质在凝胶中迁移为六个不同的峰。
The envelope of pseudorabies virions can be removed by treatment with Triton X-100, leaving the nucleocapsid intact. The viral envelope removed in this fashion contains all the glycoproteins and about 40 per cent of the proteins of the virions. Analysis by polyacrylamide gel electrophoresis revealed that the envelope proteins migrate as four distinct peaks, all of which contain glycoproteins. The proteins of the nucleocapsids migrate in the gels as six distinct peaks.