Comparative and Ontogenic Biochemistry Low Molecular Weight Components of Antioxidative Defense System in the Black Sea Mollusk Anadara Kagoshimensis Brugùiere
Comparative and Ontogenic Biochemistry Low Molecular Weight Components of Antioxidative Defense System in the Black Sea Mollusk Anadara Kagoshimensis Brugùiere
复制标题
黑海软体动物 Anadara Kagoshimensis Brugùiere 抗氧化防御系统的比较和个体生物化学低分子量成分
DOI：
--
复制
发表时间：
--
期刊：
影响因子：
--
通讯作者：
T. I. Andreenko
中科院分区：
文献类型：
--
作者：
O. L. Gostyukhina;T. I. Andreenko
研究了黑海软体动物鹿儿岛阿纳达（Anadara kagoshimensis）肝胰腺、鳃和足部还原型谷胱甘肽（GSH）水平、谷胱甘肽偶联抗氧化酶、谷胱甘肽过氧化物酶和谷胱甘肽还原酶活性以及葡萄糖、尿素和氨基酸水平。在足部检测到最高水平的谷胱甘肽和谷胱甘肽过氧化物酶的最高活性，表明谷胱甘肽在酶内和独立发挥积极的抗氧化作用。葡萄糖、氨基酸和尿素的水平在肝胰腺和鳃中最高，在足部最低。这些低分子量抗氧化剂可能参与软体动物组织抵御自由基氧化和适应低氧环境。它们的浓度与对活性氧（ROS）的抑制程度呈非线性相互关系。研究了不同LMW抗氧化物在许多双壳类动物的组织中的作用：Littorina littorea[8]、贻贝Crenomytilus grayanus[9,10]、Mytilus trossulus[11]、Mytilus galloprovincialis[3]、长牡蛎[4,10]、Crenomytilus grayanus、Modiolus kurilen-sis、Mercenaria stimpsoni、Glycymeris yessoensis、pecten Mizuhopecten yessoensis[10]。对于阿纳达拉属，这类研究较少[8,10,12,13]。这些软体动物的AO复合物是特别有趣的，因为它们的淋巴含有红细胞和血红蛋白，而软体动物对缺氧的耐受性很强，
—The level of reduced glutathione (GSH) and activity of glutathione-coupled antioxidative enzymes, glutathione peroxidase and glutathione reductase, as well as the level of glucose, carbamide and amino acids were studied in the hepatopancreas, gills and foot of the Black Sea mollusk Anadara kagoshimensis. A highest level of GSH and highest activity of glutathione peroxidase were detected in the foot indicative of an active antioxidative role glutathione plays both within the enzyme and independently. The levels of glucose, amino acids and carbamide were highest in the hepatopancreas and gills and lowest in the foot. A possible involvement and role of these low molecular weight antioxidants in the defense of mollusk tissues from free radical oxidation and in the adaptation to hypoxic habitat is considered. hibit a nonlinear interrelationship between their concentration and the degree of inhibition of re-active oxygen species (ROS) [6]. The functions of different LMW antioxidants are studied in tissues of many bivalve species: Littorina littorea [8], mussel Crenomytilus grayanus [9, 10], Mytilus trossulus [11], Mytilus galloprovincialis [3], Crassostrea gigas [4, 10], Crenomytilus grayanus, Modiolus kurilen-sis, Mercenaria stimpsoni, Glycymeris yessoensis, pecten Mizuhopecten yessoensis [10]. For the genus Anadara, such studies are less numerous [8, 10, 12, 13]. AO complex of these mollusks is of particular interest because their he-molymph contains erythrocytes and hemoglobin, while the mollusks are very tolerant to hypoxia,