There are two C4 genetic loci and a null allele in the chimpanzee.
There are two C4 genetic loci and a null allele in the chimpanzee.
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黑猩猩中有两个 C4 遗传位点和一个无效等位基因。
DOI：
10.1007/bf00343702
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发表时间：
1987
期刊：
影响因子：
3.2
通讯作者：
Alper,CA
中科院分区：
文献类型：
--
作者：
Granados,J;Awdeh,ZL;Chen,JH;Giles,CM;Balner,H;Yunis,EJ;Alper,CA
用琼脂糖凝胶电泳技术研究了黑猩猩inc4基因多态性，用抗人C4血清和C4敏感溶血覆盖层免疫固定研究了黑猩猩inc4基因多态性。一般情况下，免疫固定模式显示多个部分重叠的条带，其中只有最阴极的条带具有强溶血活性。与人C4类似，后者被命名为C4B，而通过免疫固定检测到的溶血活性较小的被命名为C4A。黑猩猩C4A和C4B与人和小鼠（单克隆）抗C4B和人抗ch1反应，但不与单克隆抗C4A或人抗ch2、Ch3、Rg1或Rg2反应。在十二烷基硫酸钠聚丙烯酰胺凝胶电泳上，C4B α链的表观相对质量在mr93 000左右，略低于C4A。有3个C4A变异体和2个C4B变异体作为常染色体共显性遗传在家族中，作为C4A-C4B共分离对，没有可检测到的交叉。这些对遗传了黑猩猩白细胞抗原类型C2和BF变体，没有可检测到的交叉。在家族研究中观察到带有c4b *Q0的半零lc4单倍型。在可能没有亲缘关系的黑猩猩中鉴定出了NineBF、C2、C4A、C4Ballelic单倍型组合（复合体）。
Genetic polymorphism inC4in the chimpanzee was studied by agarose gel electrophoresis of desialated plasma and development of patterns by immunofixation with antiserum to human C4 and by a C4-sensitive hemolytic overlay. In general, immunofixation patterns showed multiple partially overlapping bands of which only the most cathodal had strong hemolytic activity. In analogy to human C4, the latter were designated C4B, whereas those detected by immunofixation which had little hemolytic activity were designated C4A. Chimp C4A and C4B reacted with human and mouse (monoclonal) anti-C4B and human anti-Ch1 but neither reacted with monoclonal anti-C4A or human anti-Ch2, Ch3, Rg1, or Rg2. On sodium dodecyl sulfate polyacrylamide gel electrophoresis, the alpha chain of C4B showed a slightly lower apparent relative mass than that of C4A at aroundMr93 000. There were three C4A variants and two C4B variants inherited in families as autosomal codominant traits, as C4A-C4B cosegregating pairs with no detectable crossing-over. These pairs were inherited with chimpanzee leukocyte antigen types C2 and BF variants without detectable crossing-over. Half-nullC4haplotypes withC4B*Q0 were observed in family studies. NineBF, C2, C4A, C4Ballelic haplotypic combinations (complotypes) were identified among presumably unrelated chimpanzees.