Myocardial infarction differentially alters sphingolipid levels in plasma, erythrocytes and platelets of the rat.
Myocardial infarction differentially alters sphingolipid levels in plasma, erythrocytes and platelets of the rat.
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DOI：
10.1007/s00395-012-0294-0
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发表时间：
2012-11
影响因子：
9.5
通讯作者：
Górski J
中科院分区：
文献类型：
--
作者：
Knapp M;Zendzian-Piotrowska M;Błachnio-Zabielska A;Zabielski P;Kurek K;Górski J
三种生物活性鞘脂，即1-磷酸鞘氨醇（S1P）、神经酰胺（CER）和鞘氨醇（SPH）被证明与心脏缺血/再灌注损伤有关。 S1P 是一种强大的心脏保护剂，CER 激活细胞凋亡，低剂量的 SPH 具有心脏保护作用，而高剂量则具有心脏毒性。本研究的目的是检查实验性心肌梗塞对大鼠血浆、红细胞和血小板中选定鞘脂水平的影响。通过结扎左冠状动脉在雄性 Wistar 大鼠中产生心肌梗塞。结扎后 1、6 和 24 小时从腹主动脉抽取血液。分离血浆、红细胞和血小板，并通过 Agilent 6460 三重四极杆质谱仪使用正离子电喷雾电离源和多反应监测对 S1P、1-磷酸二氢鞘氨醇 (DHS1P)、SPH、二氢鞘氨醇 (DHS) 和 CER 进行定量。梗塞降低了血浆S1P、DHS1P、SPH和DHS水平，但增加了总CER水平。红细胞中，梗死后早期 SPH 和 DHS 水平急剧升高，24 小时后下降，而 S1P、DHS1P 和总 CER 水平逐渐升高。在血小板中，每种检查化合物的水平在梗塞后 1 小时和 6 小时内大幅下降，并在 24 小时内部分恢复正常。获得的结果清楚地表明，大鼠实验性心梗引起血浆、血小板和红细胞中鞘脂代谢的深刻变化。
Three bioactive sphingolipids, namely sphingosine-1-phosphate (S1P), ceramide (CER) and sphingosine (SPH) were shown to be involved in ischemia/reperfusion injury of the heart. S1P is a powerful cardioprotectant, CER activates apoptosis and SPH in a low dose is cardioprotective whereas in a high dose is cardiotoxic. The aim of the present study was to examine effects of experimental myocardial infarction on the level of selected sphingolipids in plasma, erythrocytes and platelets in the rat. Myocardial infarction was produced in male Wistar rats by ligation of the left coronary artery. Blood was taken from the abdominal aorta at 1, 6 and 24 h after the ligation. Plasma, erythrocytes and platelets were isolated and S1P, dihydrosphingosine-1-phosphate (DHS1P), SPH, dihydrosphingosine (DHS) and CER were quantified by means of an Agilent 6460 triple quadrupole mass spectrometer using positive ion electrospray ionization source with multiple reaction monitoring. The infarction reduced the plasma level of S1P, DHS1P, SPH and DHS but increased the level of total CER. In erythrocytes, there was a sharp elevation in the level of SPH and DHS early after the infarction and a reduction after 24 h whereas the level of S1P, DHS1P and total CER gradually increased. In platelets, the level of each of the examined compounds profoundly decreased 1 and 6 h after the infarction and partially normalized in 24 h. The results obtained clearly show that experimental heart infarction in rats produces deep changes in metabolism of sphingolipids in the plasma, platelets and erythrocytes.
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影响因子：
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DOI：
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发表时间：
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影响因子：
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