Relationship between Oral Malodor and the Global Composition of Indigenous Bacterial Populations in Saliva
Relationship between Oral Malodor and the Global Composition of Indigenous Bacterial Populations in Saliva
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DOI：
10.1128/aem.02304-09
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发表时间：
2010-05-01
影响因子：
4.4
通讯作者：
Yamashita, Yoshihisa
中科院分区：
文献类型：
--
作者：
Takeshita, Toru;Suzuki, Nao;Yamashita, Yoshihisa
口腔恶臭主要来自口腔内土著细菌种群的代谢活动，但全球细菌组成的健康或口腔恶臭相关模式是否存在仍不清楚。在这项研究中，在240名受试者抱怨口腔恶臭的唾液中的细菌组成分为组的基础上的末端限制性片段长度多态性（T-RFLP）的配置文件使用层次聚类分析，和模式的微生物群落组成的那些表现出较高和较低的恶臭进行了探讨。检测到四种类型的细菌群落组成（集群I，II，III和IV）。测量口腔恶臭强度的两个参数（口腔空气中挥发性含硫化合物的浓度和感官评分）在聚类I中明显低于其他聚类。采用多变量分析，在调整了潜在的混杂因素，如细菌总数，平均牙周袋深度和舌苔评分后，口腔恶臭水平的差异有统计学意义（P < 0.001）。在具有不同比例的土著成员的四个簇中，簇I的T-RFLP谱被牵连为具有比其他簇更高比例的链球菌属、颗粒体属、罗氏菌属和密螺旋体属物种的细菌群体。这些结果清楚地表明了本地细菌种群的全球组成与口腔恶臭的严重程度之间的相关性。
Oral malodor develops mostly from the metabolic activities of indigenous bacterial populations within the oral cavity, but whether healthy or oral malodor-related patterns of the global bacterial composition exist remains unclear. In this study, the bacterial compositions in the saliva of 240 subjects complaining of oral malodor were divided into groups based on terminal-restriction fragment length polymorphism (T-RFLP) profiles using hierarchical cluster analysis, and the patterns of the microbial community composition of those exhibiting higher and lower malodor were explored. Four types of bacterial community compositions were detected (clusters I, II, III, and IV). Two parameters for measuring oral malodor intensity (the concentration of volatile sulfur compounds in mouth air and the organoleptic score) were noticeably lower in cluster I than in the other clusters. Using multivariate analysis, the differences in the levels of oral malodor were significant after adjustment for potential confounding factors such as total bacterial count, mean periodontal pocket depth, and tongue coating score (P < 0.001). Among the four clusters with different proportions of indigenous members, the T-RFLP profiles of cluster I were implicated as the bacterial populations with higher proportions of Streptococcus, Granulicatella, Rothia, and Treponema species than those of the other clusters. These results clearly correlate the global composition of indigenous bacterial populations with the severity of oral malodor.