Vascular endothelial growth factor and platelet-derived endothelial cell growth factor expression are implicated in the angiogenesis of endometrial cancer
Vascular endothelial growth factor and platelet-derived endothelial cell growth factor expression are implicated in the angiogenesis of endometrial cancer
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DOI：
10.1016/s0959-8049(99)00201-4
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发表时间：
2000-01-01
影响因子：
8.4
通讯作者：
Kudo, T
中科院分区：
文献类型：
--
作者：
Seki, N;Kodama, J;Kudo, T
尽管已经描述了许多血管生成因子，但尚不清楚哪些因子在子宫内膜癌中表达。本研究的目的是检查两种血管生成因子的 mRNA 水平。子宫内膜癌组织中的血管内皮生长因子（VEGF）和血小板源性内皮细胞生长因子（PD-ECGF）及其与包括微血管密度在内的临床病理特征的关系。使用β-肌动蛋白作为内标，通过半定量逆转录聚合酶链反应评估 38 名子宫内膜癌患者的 VEGF 和 PD-ECGF mRNA 水平。还通过标本血管最多的区域中因子 VIII 相关抗原的免疫染色来评估微血管计数。非子宫内膜样肿瘤的VEGF/β-actin比值显着高于子宫内膜样肿瘤（P = 0.013）。淋巴管腔受累病例的VEGF/β-actin比值显着高于无淋巴管腔受累病例（P=0.021）。尽管没有统计学意义，但 3 级肿瘤中的 PD-ECGF/β-肌动蛋白比率高于 1 级和 2 级肿瘤（P = 0.066）。微血管密度与VEGF和PD-ECGF mRNA表达水平显着相关（分别为P = 0.041和P < 0.0001）。我们的研究结果证明 VEGF 和 PD-ECGF 的表达均参与促进子宫内膜癌的血管生成。此外，VEGF和PD-ECGF可能通过诱导血管生成而增强高级别肿瘤或某些预后不良的组织学亚型的侵袭潜力。 (C) 2000 Elsevier Science Ltd. 保留所有权利。
Although many angiogenic factors have been described, it is not well defined which factors are expressed in endometrial cancer. The object of this study was to examine mRNA levels of the two angiogenic factors. vascular endothelial growth factor (VEGF) and platelet-derived endothelial cell growth factor (PD-ECGF) in endometrial cancer tissues and their association with clinicopathological features including microvessel density. The level of VEGF and PD-ECGF mRNAs was assessed by semi-quantitative reverse transcription-polymerase chain reaction using beta-actin as an internal standard in 38 patients with endometrial cancer. Microvessel counts were also assessed by immunostaining for factor VIII-related antigen in the most vascularised area of the specimen. VEGF/beta-actin ratios of non-endometrioid tumours were significantly higher than those of endometrioid tumours (P = 0.013). VEGF/beta-actin ratios of cases with lymph-vascular space involvement were significantly higher than those of cases without lymph-vascular space involvement (P = 0.021). Although it was not statistically significant, PD-ECGF/beta-actin ratios in grade 3 tumours were higher than those in grade 1 and 2 tumours (P = 0.066). The microvessel density was significantly correlated with the level of VEGF and PD-ECGF mRNA expression (P = 0.041 and P < 0.0001, respectively). Our findings provide evidence that the expression of both VEGF and PD-ECGF is involved in the promotion of angiogenesis in endometrial cancer. In addition, VEGF and PD-ECGF might contribute to the aggressive potential of high grade tumours or certain histological subtypes with unfavourable prognosis through the induction of angiogenesis. (C) 2000 Elsevier Science Ltd. All rights reserved.