Characterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells
Characterization and phenotypic variation with passage number of cultured human endometrial adenocarcinoma cells
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DOI：
10.1016/j.tice.2007.09.007
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发表时间：
2008-04-01
期刊：
影响因子：
2.6
通讯作者：
Owen, Gareth I.
中科院分区：
文献类型：
--
作者：
Kato, Sumie;Espinoza, Natalia;Owen, Gareth I.
尽管有许多子宫内膜癌细胞系，但对原代培养的子宫内膜肿瘤的进展和转移知之甚少。在此，在原代培养中维持I~III期子宫内膜腺癌，并观察其表型和蛋白表达的变化与传代次数的关系。在早期传代时，培养的人子宫内膜癌(CHEC)细胞显示典型的上皮细胞形态，成群生长在腺体结构中，细胞角蛋白染色阳性。然而，随着传代次数的增加，CHEC细胞的形态发生了变化，表现为间质表型，并伴随着细胞角蛋白的显著减少，以及Ut-肌动蛋白和波形蛋白的表达增加。从原始肿瘤分离的间质细胞同时培养，未能显示出相同的形态特征或蛋白质表达模式。我们通过筛选癌症相关蛋白，包括小窝蛋白-1和组织因子，与已建立的癌细胞株和相应的非癌症细胞进行比较，进一步确定了CHEC细胞的特征。这份报告表明，培养的子宫内膜腺癌细胞可以经历上皮间充质转化的表型和蛋白表达的变化。这项工作表明，原发肿瘤和显示间质形态的细胞系可能从腺癌起源经历了上皮-间充质转化。(C)2007爱思唯尔有限公司。保留所有权利。
Despite numerous endometrial cancer cell lines, little is know about the progression and transition of primary cultured endometrial tumours. Herein, a stage I grade III endometrial adenocarcinoma was maintained in primary culture and the phenotypic and protein expression changes were observed in relation to passage number. At early passage numbers, cultured human endometrial cancer (CHEC) cells displayed classic eprithelial cell morphology, growing in groups in a glandular structure and staining positive for cytokeratin. However, with increasing passage number, CHEC cells changed in morphology to display a stromal phenotype which was accompanied by a significant reduction in cytokeratin and increases in ut-actin and vimentin expression. Simultaneous culture of stromal cells isolated from the original tumour failed to show the same morphological characteristics or protein expression patterns. We further characterised CHEC cells through a screening of cancer related proteins, among others, caveolin-1 and Tissue factor in comparison with established cancer cell lines and corresponding non-cancerous cells. This report demonstrates that endometrial adenocarcinoma cells in culture can undergo phenotypic and protein expression changes reminiscent of epithelial-mesenchymal transition. This work suggests that primary tumours and cell lines displaying stromal morphologies may have undergone epithelial-mesenchymal transition from an adenocarcinoma origin. (C) 2007 Elsevier Ltd. All rights reserved.