SPONTANEOUS NEURONAL FIRING PATTERNS IN FETAL-RAT CORTICAL NETWORKS DURING DEVELOPMENT INVITRO - A QUANTITATIVE-ANALYSIS
SPONTANEOUS NEURONAL FIRING PATTERNS IN FETAL-RAT CORTICAL NETWORKS DURING DEVELOPMENT INVITRO - A QUANTITATIVE-ANALYSIS
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DOI：
10.1007/bf00247027
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发表时间：
1987-01-01
影响因子：
2
通讯作者：
CORNER, MA
中科院分区：
文献类型：
--
作者：
HABETS, AMMC;VANDONGEN, AMJ;CORNER, MA
在用 19 日龄大鼠胎儿制备的分离枕叶皮层培养物中研究了自发生物电活动 (SBA) 的发展。所有培养物在体外 (div) 5 至 33 天期间每天记录一次，均显示 SBA。根据平稳性标准选择后，对 76 个细胞外记录的单单位尖峰序列进行计算机分析。在（i）峰间间隔依赖性和（ii）平均放电率波动（大约一分钟或更长时间）中发现了统计上显着的发展趋势。高度依赖的放电模式，包括刻板的爆发，主要出现在 9-12 格组中，而放电强度的每分钟波动在最古老的组（22-33 格）中比在年轻文化中更为明显。此外，基于因子分析定义的放电类别表明，这种波动几乎只出现在以明显“爆发”方式放电的神经元中，而不是以相对连续的方式放电的神经元中。在12 div之前，仅在电子显微镜下观察到一些成熟的突触结构，但此后数量稳定增加。 14 div 之前没有任何培养物，但所有早于此的培养物均因谷氨酸脱羧酶的存在而染色呈阳性。体外三周后，出现了广泛的免疫反应性、推定的 GABA 能网络。
The development of spontaneous bioelectric activity (SBA) was studied in dissociated occipital cortex cultures prepared with 19 day old rat fetuses. All cultures, recorded one per diem from 5 to 33 days in vitro (div), showed SBA. Computer analysis of 76 extracellularly recorded single unit spike trains was carried out after selection on the basis of stationarity criteria. Statistically significant development trends were found in (i) interspike interval dependencies and (ii) fluctuations in mean firing rate, on the order of a minute or longer. The highly dependent firing patterns, including stereotyped bursting, were present mostly in the 9-12 div group, whereas minute-to-minute fluctuations in the intensity of firing were considerably more pronounced in the oldest group (22 - 33 div) than in the younger cultures. In addition, firing categories defined on the basis of factor-analysis revealed that such fluctuations were almost exclusively to be found in neurons which fired in a pronounced ''burst'', rather than a relatively continuous fashion. Only a few mature appearing synaptic structures were observed electron microscopically prior to 12 div, but increased steadily in number thereafter. No cultures prior to 14 div, but all cultures older than this, stained positively for the presence of glutamic acid decarboxylase. An extensive immunoreactive, putative GABAergic, network was present by three weeks in vitro.