Vessel painting of the micro circulation using fluorescent lipophilic tracers
Vessel painting of the micro circulation using fluorescent lipophilic tracers
复制标题
DOI：
10.1016/j.mvr.2005.06.002
复制
发表时间：
2005-07-01
影响因子：
3.1
通讯作者：
Konerding, MA
中科院分区：
文献类型：
--
作者：
Ravnic, DJ;Jiang, XQ;Konerding, MA
灵活的方法来定义微血管形态学是有用的，在急性和慢性微循环结构变化的研究。在这份报告中，我们研究了效用的血管内输注的亲脂性碳菁示踪剂的结构评估的视网膜，皮肤，肺和结肠微循环。微血管标记技术，这里称为荧光血管绘画，涉及磺化亲脂性碳菁示踪剂的血管内注射。使用腐蚀铸造和二维和三维扫描电子显微镜的形态测量比较，评估血管绘画在形态测量中的效用。比较表明，荧光血管绘画适度高估了分支间的角度，分支间的距离，和血管直径的2D粘膜丛的结肠。这些差异缩小与共聚焦显微镜的应用。荧光血管涂抹的优点包括（1）填充所有组织，包括小鼠皮肤的相对高阻力微血管，（2）能够使用组织复染剂如DAPI，和（3）醛固定后亲脂性示踪剂的长期稳定性。这些研究表明，在微循环的形态测定研究中，荧光血管绘画作为腐蚀铸造的补充技术的实用性。(c)2005年爱思唯尔公司All rights reserved.
Flexible approaches to defining microvessel morphometry are useful in the study of both acute and chronic structural changes of the microcirculation. In this report, we examined the utility of the intravascular infusion of lipophilic carbocyanine tracers in the structural assessment of the retina, skin, lung, and colon microcirculation. The microvessel labeling technique, here termed fluorescent vessel painting, involved the intravascular injection of sulfonated lipophilic carbocyanine tracers. The utility of vessel painting in morphometry was assessed using morphometric comparisons with corrosion casting and 2-dimensional and 3-dimensional scanning electron microscopy. The comparisons demonstrated that fluorescent vessel painting modestly overestimated the interbranch angles, interbranch distances, and vessel diameters of the 2D mucosal plexus of the colon. These differences were narrowed with the application of confocal microscopy. The advantages of fluorescence vessel painting included (1) the filling of all tissues including the relatively high resistance microvessels of the mouse skin, (2) the ability to use tissue counterstains such as DAPI, and (3) the prolonged stability of the lipophilic tracer after aldehyde fixation. These studies suggest the utility of fluorescent vessel painting as a complementary technique to corrosion casting in the morphometric study of the microcirculation. (c) 2005 Elsevier Inc. All rights reserved.