MACROPHAGES OF HEMANGIOBLASTIC LINEAGE INVADE THE LENS VESICLE-ECTODERM INTERSPACE DURING CLOSURE AND DETACHMENT OF THE AVIAN EMBRYONIC LENS
MACROPHAGES OF HEMANGIOBLASTIC LINEAGE INVADE THE LENS VESICLE-ECTODERM INTERSPACE DURING CLOSURE AND DETACHMENT OF THE AVIAN EMBRYONIC LENS
复制标题
DOI：
10.1007/bf00678718
复制
发表时间：
1991-10-01
影响因子：
3.6
通讯作者：
NAVASCUES, J
中科院分区：
文献类型：
--
作者：
CUADROS, MA;MARTIN, C;NAVASCUES, J
在透镜原基从头部外胚层闭合和分离期间，在透镜囊泡边缘和透镜柄中有明显的细胞死亡区域。与该细胞死亡区域密切相关的游离吞噬细胞被解释为从透镜上皮迁移的细胞。用扫描电镜、透射电镜、酸性磷酸酶组织化学染色和免疫组化方法对鸡胚和鹌鹑卵黄囊嵌合体的透镜囊泡相关吞噬细胞进行了分析。这些细胞具有与其他胚胎组织中的巨噬细胞相同的形态特征。与吞噬细胞碎片的上皮细胞相反，它们表现出强酸性磷酸酶活性，这是巨噬细胞的典型特征。此外，在鸡胚-鹌鹑卵黄囊嵌合体中，游离吞噬细胞呈MB 1阳性，因此它们来自卵黄囊中的成血管细胞谱系。这些结果表明，透镜囊泡相关的游离吞噬细胞是巨噬细胞。在视网膜旁间充质和视杯边缘的MB 1阳性阿米巴样细胞的观察表明，这些巨噬细胞通过眼原基周围的间充质迁移。在透镜囊泡和外胚层之间的间隙和透镜的内腔以及外胚层和透镜上皮内均可见大颗粒。在这些位置，它们去除细胞碎片，从而有助于细胞死亡区域的完全消失。巨噬细胞保留在透镜囊泡-外胚层间隙中，直到发育阶段被角膜内皮细胞侵入。
An area of cell death is apparent in the lens vesicle margin and the lens stalk during closure and detachment of the lens anlage from the cephalic ectoderm. Free phagocytic cells closely associated with this area of cell death have been interpreted as cells migrating from the lens epithelium. Scanning and transmission electron microscopy, light-microscopic histochemical staining for acid phosphatase and immunostaining using MB1 (a monoclonal antibody specific for quail endothelial and hemopoietic cells) of chimeras of chick embryo and quail yolk sac were used to analyze these lens vesicle-associated free phagocytic cells. The cells have morphological features identical to those of macrophages in other embryonic tissues. In contrast to epithelial cells phagocytosing cell debris, they exhibit strong acid phosphatase activity, a feature typical of macrophages. In addition, free phagocytic cells are MB1 positive in chick embryo-quail yolk sac chimeras, hence they proceed from cells of hemangioblastic lineage originating in the yolk sac. These results indicate that the lens vesicle-associated free phagocytic cells are macrophages. Observations of MB1 positive amoeboid cells in the juxta-retinal mesenchyme and on the borders of the optic cup suggest that these macrophages migrate through the mesenchyme surrounding the eye primordium. Macrophages are seen in both the interspace between lens vesicle and ectoderm and in the lumen of the lens as well as within both the ectoderm and the lens epithelium. In these locations they remove cell debris, and thereby contribute to the complete disappearance of the area of cell death. Macrophages remain in the lens vesicle-ectoderm interspace until developmental stages at which it is invaded by corneal endothelial cells.