Arabidopsis thaliana NOP10 is required for gametophyte formation
Arabidopsis thaliana NOP10 is required for gametophyte formation
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拟南芥 NOP10 是配子体形成所必需的
DOI：
10.1111/jipb.12652
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发表时间：
2018
影响因子：
11.4
通讯作者：
Zhang Xue-Qin
中科院分区：
文献类型：
--
作者：
Li Lin-Xiao;Liao Hong-Ze;Jiang Li-Xi;Tan Qing;Ye De;Zhang Xue-Qin
雌配子体是高等植物有性生殖的关键，但其发育的分子机制尚不清楚。本文报道拟南芥（Arabidopsis thaliana）的NOP 10（AtNOP 10）是雌配子体形成所必需的。AtNOP 10主要在幼苗和生殖组织中表达，包括花药、花粉粒和胚珠。AtNOP 10的突变在发育早期中断了功能性大孢子的有丝分裂，并阻止了胚囊中的极核融合。AtNOP 10与酿酒酵母NOP 10（Saccharomycescerevisiae，ScNOP 10）的氨基酸序列高度相似，ScNOP 10是H/ACA核仁小核糖核蛋白颗粒（H/ACA snoRNP）的重要组成部分，参与18 SrRNA的合成和rRNA的假尿苷化。ScNOP 10的异源表达补充了Atnop 10的突变表型。因此，AtNOP 10在拟南芥配子体形成过程中影响功能性大孢子有丝分裂和极核融合。
The female gametophyte is crucial for sexual reproduction of higher plants, yet little is known about the molecular mechanisms underlying its development. Here, we report thatArabidopsis thaliana NOP10(AtNOP10) is required for female gametophyte formation. AtNOP10 was expressed predominantly in the seedling and reproductive tissues, including anthers, pollen grains, and ovules. Mutations inAtNOP10interrupted mitosis of the functional megaspore during early development and prevented polar nuclear fusion in the embryo sacs. AtNOP10 shares a high level of amino acid sequence similarity withSaccharomyces cerevisiae(yeast) NOP10 (ScNOP10), an important component of the H/ACA small nucleolar ribonucleoprotein particles (H/ACA snoRNPs) implicated in 18S rRNA synthesis and rRNA pseudouridylation. Heterologous expression ofScNOP10complemented the mutant phenotype ofAtnop10. Thus, AtNOP10 influences functional megaspore mitosis and polar nuclear fusion during gametophyte formation inArabidopsis.