Expression of F4, 8.12, 3I, and 16/6 anti-DNA idiotype-related antigens on cationic human IgG myeloma proteins.
Expression of F4, 8.12, 3I, and 16/6 anti-DNA idiotype-related antigens on cationic human IgG myeloma proteins.
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F4、8.12、3I 和 16/6 抗 DNA 独特型相关抗原在阳离子人 IgG 骨髓瘤蛋白上的表达。
DOI：
10.1006/clin.1994.1190
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
R. Kyle
中科院分区：
文献类型：
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作者：
R. Williams;A. Schriber;C. Malone;F. Silvestris;N. Hannigan;M. Klein;C. Namyst;R. Kyle
对100个阳离子分离的人骨髓瘤IgG蛋白进行了四种抗dna独特型（Id）标记F4、3I、8.12和16/6的表达研究。100例骨髓瘤中有43例显示至少存在一种抗dna独特型。F4阳性27例，16/6阳性18例，3I阳性8例，8.12阳性7例。在相同的14种骨髓瘤蛋白上发现了两种不同的抗dna独特型标记，3种骨髓瘤显示了三种不同的抗dna标记。100个骨髓瘤中只有1个发现了抗dna活性，但与抗dna Id的存在无关。10个骨髓瘤显示抗f (ab')2活性，其中6个也显示存在抗dna Id标记。当将表达抗dna id的骨髓瘤蛋白与已知id阳性的人IgG抗dna抗体在直接竞争elisa中进行比较时，与已知的人抗dna抗体相比，记录到的抑制要小得多。我们的研究结果表明，人IgG骨髓瘤蛋白可能与抗dna独特型抗体呈阳性反应，但不表达完整的抗dna独特型抗原复合物。
One hundred cationic isolated human IgG myeloma proteins were studied for expression of four anti-DNA idiotypic (Id) markers F4, 3I, 8.12, and 16/6. Forty-three of 100 myelomas showed the presence of at least one anti-DNA idiotype. Twenty-seven were F4 positive, 18 were 16/6 positive, 8 were 3I positive, and 7 were positive for 8.12. Two different anti-DNA idiotypic markers were found together on the same 14 myeloma proteins and 3 myelomas showed three different anti-DNA Ids. Anti-DNA activity was found on only 1 of 100 myelomas but not associated with presence of an anti-DNA Id. Ten myelomas showed anti-F(ab')2 activity and 6 of these also showed the presence of an anti-DNA Id marker. When myeloma proteins expressing anti-DNA Ids were compared in direct competition ELISAs with known Id-positive human IgG anti-DNA antibodies, much less inhibition was recorded in comparison to known human anti-DNA antibodies. Our findings indicate that human IgG myeloma proteins may show positive reactions with anti-DNA idiotypic antibodies but do not express the complete anti-DNA idiotypic antigenic complex.