Prognostic role of CD10+ myeloid cells in association with tumor budding at the invasion front of colorectal cancer
Prognostic role of CD10+ myeloid cells in association with tumor budding at the invasion front of colorectal cancer
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DOI：
10.1111/j.1349-7006.2011.01987.x
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发表时间：
2011-09-01
期刊：
影响因子：
5.7
通讯作者：
Tani, Tohru
中科院分区：
文献类型：
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作者：
Do Trong Khanh;Mekata, Eiji;Tani, Tohru
CD 10在肿瘤细胞中的表达已被报道与结直肠癌（CRC）的肝转移相关。然而，在肿瘤侵袭前沿的成纤维细胞和CD 10阳性免疫细胞尚未得到全面研究。我们将CD 10表达模式分为三种类型的细胞：肿瘤细胞（tCD 10）、间质肌成纤维细胞（sCD 10）和免疫细胞（iCD 10），并研究了它们与转化生长因子-β（TGF-β 1）蛋白表达的相关性和肿瘤萌芽级别。对几种细胞表面标志物进行染色，以检测iCD 10（+）细胞的表型，包括CD 3、CD 20、CD 11b、CD 14、CD 15和CD 163。对206例结直肠癌患者的标本和随访资料进行了检查。在多变量分析中，iCD 10可能是I-III期CRC无复发生存期和总生存期的独立预后因素（风险比分别为2.522 [1.299-4.896]，P = 0.006; 2.890 [1.357-6.157]，P = 0.006）。sCD 10和iCD 10的表达与肿瘤细胞中TGF-β 1的表达和肿瘤出芽分级密切相关。iCD 10（+）细胞的表型为CD 11b（+）和CD 15（+）粒细胞。sCD 10（+）成纤维细胞和iCD 10（+）粒细胞在肿瘤浸润前沿的浸润可能与TGF-β 1蛋白表达相互作用，增强肿瘤出芽分级。iCD 10在肿瘤浸润前沿的表达水平代表了I-III期CRC的独立预后生物标志物，并可整合到新的分期系统中。（Cancer Sci 2011; 102：1724-1733）
The expression of CD10 in tumor cells has been reported to correlate with liver metastasis in colorectal cancer (CRC). However, fibroblasts and immune cells positive for CD10 at the tumor invasion front have not been comprehensively studied. We classified CD10 expression patterns into three types of cells, tumor cells (tCD10), stromal myofibroblasts (sCD10), and immune cells (iCD10), and investigated their correlation with the expression of transforming growth factor-beta (TGF-beta 1) protein and tumor budding grade. Several cell surface markers were stained to detect the phenotype of iCD10(+) cells, including CD3, CD20, CD11b, CD14, CD15, and CD163. Specimens and follow-up data of 206 CRC patients were examined. In multivariate analysis, iCD10 could be an independent prognostic factor for both recurrence-free survival and overall survival in stage I-III CRC (hazard ratio, 2.522 [1.299-4.896], P = 0.006; 2.890 [1.357-6.157], P = 0.006, respectively). The expression of sCD10 and iCD10 was strongly correlated with TGF-beta 1 expression in tumor cells and tumor budding grade. The phenotype of iCD10(+) cells was CD11b(+) and CD15(+) granulocytes. The infiltration of sCD10(+) fibroblasts and iCD10(+) granulocytes at the tumor invasion front might interact with TGF-beta 1 protein expression and enhance tumor budding grade. The expression level of iCD10 at the tumor invasion front represented an independent prognostic bio-marker in stage I-III CRC and could be integrated into a new staging system. (Cancer Sci 2011; 102: 1724-1733)