THE INHIBITORY EFFECTS OF OKADAIC ACID ON PLATELET-FUNCTION
THE INHIBITORY EFFECTS OF OKADAIC ACID ON PLATELET-FUNCTION
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DOI：
10.1016/0014-5793(92)80768-c
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发表时间：
1992-07-28
期刊：
影响因子：
3.5
通讯作者：
IKEBE, M
中科院分区：
文献类型：
--
作者：
HIGASHIHARA, M;TAKAHATA, K;IKEBE, M
Okadaic acid (OA), a potent inhibitor of protein phosphatases type 1 and type 2A, inhibited thrombin-induced platelet aggregation (IC50 = 0.8-mu-M), [C-14]serotonin release and increase in intracellular Ca2+ ([Ca2+]i) in the same dose dependence. In the absence of thrombin OA increased the phosphorylation of 50-kDa protein and 20-kDa myosin light chain (MLC20). The 50-kDa protein phosphorylation was accomplished within a shorter time period and at a lower concentration than was the MLC20. OA decreased the thrombin-induced phosphorylation of 47-kDa protein and MLC20, although phosphorylation of MLC20 reincreased at higher concentrations of OA (5-10-mu-M). Since type 2A phosphatase is more sensitive to OA than type 1, these results suggest that type 2A phosphatases are involved in the regulation of Ca2+ signaling in thrombin-induced platelet activation.
