Odorant-sensitive adenylate cyclase: rapid, potent activation and desensitization in primary olfactory neuronal cultures.
Odorant-sensitive adenylate cyclase: rapid, potent activation and desensitization in primary olfactory neuronal cultures.
复制标题
气味敏感的腺苷酸环化酶：在原代嗅觉神经元培养物中快速、有效的激活和脱敏。
DOI：
10.1073/pnas.88.6.2366
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发表时间：
1991
影响因子：
11.1
通讯作者：
Snyder,SH
中科院分区：
文献类型：
--
作者：
Ronnett,GV;Parfitt,DJ;Hester,LD;Snyder,SH
使用原代嗅觉神经元培养物，我们已经证明了响应于气味剂的cAMP水平和腺苷酸环化酶[AC; ATP焦磷酸裂解酶（环化），EC 4.6.1.1]活性的快速、有效的增加。异丁基-甲氧基吡嗪在1 nM时具有活性。气味增强依赖于Ca 2+浓度，在10-100 μ M时效果最大。双相的时间和浓度相关的影响发生与所有的气味。所有的气味剂检查引起脱敏与AC反应废除气味剂后立即重新应用。当去除后1分钟重新应用时，气味剂引起的AC响应大于第一次暴露，这意味着细胞的气味剂的“记忆”。
Using primary olfactory neuronal cultures, we have demonstrated rapid, potent increases in cAMP levels and adenylate cyclase [AC; ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1] activity in response to odorants. Isobutyl-methoxypyrazine is active at 1 nM. Odorant enhancement is dependent on Ca2+ concentration with maximal effects at 10-100 microM. Biphasic temporal and concentration-related effects occur with all odorants. All odorants examined elicit desensitization with AC responses abolished when odorants are reapplied immediately after removal. When reapplied 1 min after removal, odorants elicit an AC response greater than on first exposure, implying a cellular "memory" for odorants.