MONOCLONAL-ANTIBODIES WHICH RECOGNIZE DIFFERENT CELL-TYPES IN THE RAT RETINA
MONOCLONAL-ANTIBODIES WHICH RECOGNIZE DIFFERENT CELL-TYPES IN THE RAT RETINA
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DOI：
10.1038/286231a0
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发表时间：
1980-01-01
期刊：
影响因子：
64.8
通讯作者：
BARNSTABLE, CJ
中科院分区：
文献类型：
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作者：
BARNSTABLE, CJ
针对成年大鼠视网膜的膜制备物生产了七种单克隆抗体[来源于小鼠肿瘤P3-NSI 1-Ag 4 -1细胞、脾细胞杂交瘤或小鼠骨髓瘤8653细胞脾细胞杂交瘤]。以下3种抗体与大鼠感光细胞表面的特定区域反应：RET-P1标记细胞体以及外节和内节（视杆细胞而非视锥细胞），RET-P2仅标记外节，RET-P3仅标记细胞体。抗体（3）与胶质细胞反应; RET-G2和RET-G3对Mueller细胞特异，RET-G1标记脑中的胶质细胞。第7种抗体（RET-N1）与多种类型的神经元细胞反应。
Seven monoclonal antibodies [derived from mouse neoplastic P3-NSI1-Ag4-1 cell, spleen cell hybridoma or mouse myeloma 8653 cell spleen cell hybridoma] were produced against a membrane preparation from adult rat retina. The following 3 antibodies reacted with particular regions of rat photoreceptor cell surfaces: RET-P1 labeled the cell bodies, and the outer and inner segments (rods but not cones), RET-P2 labeled only outer segments and RET-P3 labeled only the cell bodies. Antibodies (3) reacted with glial cells; RET-G2 and RET-G3 were specific for Mueller cells, RET-G1 labeled glia in the brain. The 7th antibody (RET-N1) reacted with many types of neuronal cells.