The Chlamydia protease CPAF: caution, precautions and function.
The Chlamydia protease CPAF: caution, precautions and function.
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DOI：
10.1111/2049-632x.12213
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发表时间：
2014-10
影响因子：
3.3
通讯作者：
Sütterlin C
中科院分区：
文献类型：
--
作者：
Tan M;Sütterlin C
最近的生化和遗传学研究对衣原体蛋白酶CPAF的许多功能提出了质疑（Zhong et al., 2001）。2012年，我们证明了在制备衣原体感染细胞的裂解物时，多种报道的底物的蛋白水解发生在制备过程中，而不是在完整的细胞中（Chen et al., 2012）。Snavely等人最近关于CPAF零突变体的报道提供了遗传学证据，证明CPAF对于许多衣原体诱导的表型和成功的细胞内感染都不是必需的（Bavoil & Byrne, 2014； Snavely等人，2014）。
Recent biochemical and genetic studies have called into question many of the functions proposed for the chlamydial protease CPAF (Zhong et al., 2001). In 2012, we demonstrated that the proteolysis of multiple reported substrates was occurring during the preparation of lysates from Chlamydia-infected cells rather than in intact cells (Chen et al., 2012). The recent report by Snavely et al. of a CPAF null mutant now provides genetic evidence that CPAF is not necessary for many Chlamydia-induced phenotypes nor for a successful intracellular infection (Bavoil & Byrne, 2014; Snavely et al., 2014).