Glycomic profiling of invasive and non-invasive breast cancer cells
Glycomic profiling of invasive and non-invasive breast cancer cells
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DOI：
10.1007/s10719-008-9170-4
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Novotny, Milos V.
中科院分区：
文献类型：
--
作者：
Goetz, John A.;Mechref, Yehia;Novotny, Milos V.
具有不同结构的聚糖的定量分析对于更好地理解与恶性转化和癌细胞的潜在异常糖基化相关的细胞粘附现象是必不可少的。使用最近开发的糖组学技术和质谱测量，我们比较了N-连接和O-连接的寡糖配置文件不同的乳腺癌细胞系与正常上皮细胞。某些中性、唾液酸化和岩藻糖基化结构的统计学显著差异通过定量测量很容易辨别，表明区分侵袭性和非侵袭性癌症属性的潜力。相应地使用主成分分析对糖组学谱数据进行聚类，从而验证由于正常细胞系和癌细胞系之间的差异而导致的进一步的糖生物学差异。
Quantitative profiling of glycans with different structures appears essential for a better understanding of the cellular adhesion phenomena associated with malignant transformation and the underlying aberrant glycosylation of cancer cells. Using the recently developed glycomic techniques and mass-spectrometric measurements, we compare the N-linked and O-linked oligosaccharide profiles for different breast cancer cell lines with those of normal epithelial cells. Statistically significant differences in certain neutral, sialylated and fucosylated structures are readily discerned through quantitative measurements, indicating a potential of distinguishing invasive and non-invasive cancer attributes. The glycomic profile data cluster accordingly using Principal Component Analysis, verifying further glycobiological differences due to the differences between normal and cancer cell lines.