Drosophila melanogaster contains a single calmodulin gene. Further structure and expression studies.
Drosophila melanogaster contains a single calmodulin gene. Further structure and expression studies.
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果蝇含有一个钙调蛋白基因。
DOI：
10.1016/s0022-2836(05)80245-1
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发表时间：
1990
影响因子：
5.6
通讯作者：
Beckingham,K
中科院分区：
文献类型：
--
作者：
Doyle,KE;Kovalick,GE;Lee,E;Beckingham,K
我们之前已经鉴定了黑腹果蝇的钙调蛋白基因。在高等脊椎动物中，存在一个编码钙调蛋白的多基因系统，在至少一个无脊椎动物物种中，存在编码高度相关的钙调蛋白亚型的基因。因此，我们在inD中寻找了额外的钙调蛋白基因。黑猩猩。虽然我们的搜索足够灵敏，可以检测到编码钙调蛋白家族蛋白的相对差异的基因，但我们无法检测到任何额外的钙调蛋白本身基因。白纹伊蚊单个钙调蛋白基因结构和表达的进一步研究。黑色素学家已经证实，该基因包含一个微小的额外5‘外显子，仅编码5’前导的50个残基。在3‘端的测序已经证实，来自该基因的两种转录本大小类别是作为替代的多聚腺苷酸化位点使用的结果。在整个生命周期中，这两种大小类别的mRNAs的相对丰度不同，表明多聚腺苷化位点使用的发育调节。
We have previously characterized a calmodulin gene from the organismDrosophila melanogaster. In the higher vertebrates a multi-gene system for encoding calmodulin is present and, in at least one invertebrate species, genes encoding highly related calmodulin isotypes exist. We have therefore searched for additional calmodulin genes withinD. melanogaster. Although our searches were sensitive enough to detect a relatively divergent gene encoding a calmodulin family protein, we were unable to detect any additional genes for calmodulinper se. Further studies of the structure and expression of the single calmodulin gene ofD. melanogasterhave established that the gene contains a tiny additional 5′ exon encoding only 50 residues of the 5′ leader. Sequencing at the 3′ terminus has established that the two transcript size classes derived from the gene are produced as a result of alternative polyadenylation site usage. The relative abundance of the two size classes of mRNAs differs throughout the life cycle, indicating developmental regulation of polyadenylation site usage.