Comparison of the leader sequences of four group A streptococcal M protein genes.
Comparison of the leader sequences of four group A streptococcal M protein genes.
复制标题
四种 A 组链球菌 M 蛋白基因前导序列的比较。
DOI：
10.1093/nar/16.10.4667
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发表时间：
1988
影响因子：
14.9
通讯作者：
Cleary,P
中科院分区：
文献类型：
--
作者：
Haanes-Fritz,E;Kraus,W;Burdett,V;Dale,JB;Beachey,EH;Cleary,P
比较了编码1、12、24和6 M型蛋白的基因的5′部分和侧翼序列。虽然编码成熟M蛋白氨基末端的DNA序列没有明显的相似性，但上游序列和编码4个M蛋白基因的信号肽（先导序列）的DNA序列具有相当大的相似性。虽然M6和M24的前导序列具有相同的3′端，但总的来说，所有前导序列的5′端比3′端更保守。推导出的4种信号肽氨基酸序列之间的序列相似性比相应的DNA序列更广泛。我们发现，这四个序列之间严格的DNA相似性只延伸到信号肽亲水性氨基末端区域的末端，但氨基酸序列的保守性持续到各自疏水核心的末端。除M6和M24序列外，信号肽酶裂解位点附近的区域变化很大。
The 5′ portions and flanking sequences of genes encoding types 1, 12, 24, and 6 M proteins were compared. Although the DNA sequences encoding the amino-termini of the mature M proteins had no obvious similarity, upstream sequences, and those encoding the signal peptides (leader sequences) of the four M protein genes had considerable similarity. In general, the 5′ ends of all the leader sequences were more conserved than the 3′ ends, although the M6 and M24 leader sequences had identical 3′ends. Sequence similarity among the deduced amino acid sequences of the four signal peptides was more extensive than the corresponding DNA sequences. We found that strict DNA similarity among all four sequences extended only to the ends of the hydrophilic amino-terminal regions of the signal peptides, but that amino acid sequence conservation continued to the ends of the respective hydrophobic cores. With the exception of the M6 and M24 sequences, the regions adjacent to the signal peptidase cleavage sites were highly variable.