ROLE OF LYSOSOMES IN REGULATING OF VITELLOGENIN SECRETION IN THE MOSQUITO FAT-BODY
ROLE OF LYSOSOMES IN REGULATING OF VITELLOGENIN SECRETION IN THE MOSQUITO FAT-BODY
复制标题
DOI：
10.1016/0022-1910(86)90088-0
复制
发表时间：
1986-01-01
影响因子：
2.2
通讯作者：
RAIKHEL, AS
中科院分区：
文献类型：
--
作者：
RAIKHEL, AS
蚊子脂肪体的滋养细胞产生大量的卵黄蛋白前体卵黄蛋白原，然后由发育中的卵母细胞积累。卵黄原合成是一个高度调控的过程，根据生殖周期的阶段开启和关闭；吸血后27小时达到最大，然后迅速下降。在这里，我报告了一种涉及卵黄蛋白原分泌的细胞调节的新机制，例如卵黄原蛋白的溶酶体分解。四种溶酶体酶的活性在吸血后约30小时急剧上升，并在接下来的12小时内保持较高水平。视频增强荧光，结合免疫标记对卵黄蛋白原的可视化和吖啶橙对溶酶体的可视化，证明这种强烈的溶酶体活性针对卵黄蛋白原的特异性降解。后者在细胞质中减少并集中在大溶酶体中，直到后来才从滋养细胞中完全消失。
Trophocytes of the mosquito fat body produce large amounts of the yolk-protein precursor, vitellogenin, that is then accumulated by developing oocytes. Vitellogenin synthesis is a highly regulated process and is turned on and off according to the stage of the reproductive cycle; it is maximal 27 h after blood feeding and then rapidly declines. Here, I report a new mechanism involved in cellular regulation of vitellogenin secretion, e.g. the lysosomal breakdown of vitellogenin. The activities of four lysosomal enzymes rose sharply about 30 h after blood-feeding and remained high for the next 12 h. Video-enhanced fluorescence, combined with visualization of vitellogenin by immunolabelling and of lysosomes by acridine orange, demonstrated that this intense lysosomal activity was directed toward the specific degradation of vitellogenin; the latter diminished in the cytoplasm and was concentrated in large lysosomes, only later did it disappear entirely from the trophocytes.