Steroids from Marine-Derived Fungi: Evaluation of Antiproliferative and Antimicrobial Activities of Eburicol
Steroids from Marine-Derived Fungi: Evaluation of Antiproliferative and Antimicrobial Activities of Eburicol
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DOI：
10.3390/md17060372
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发表时间：
2019-06-01
期刊：
影响因子：
5.4
通讯作者：
Wielgosz-Collin, Gaetane
中科院分区：
文献类型：
--
作者：
Dias, Ana Camila Dos Santos;Couzinet-Mossion, Aurelie;Wielgosz-Collin, Gaetane
真菌中最常见的固醇是麦角固醇，其经常在人类致病真菌菌株中进行研究。这种甾醇和其他从真菌菌株中分离出来的甾醇也表现出对癌细胞系的细胞毒性和抗菌活性。海洋真菌可以产生大量的生物活性化合物。因此，本研究采用GC/MS技术对19株海洋真菌的甾醇组成进行了初步筛选，发现麦角甾醇始终是海洋真菌的主要甾醇。选择一种菌株，玫瑰粘帚霉MMS 1090，由于其高含量的埃布里考，并在七种培养基上进行一种菌株多种化合物的方法以优化其生产。在通过NMR纯化和结构鉴定后，评价了依布里考对四种癌细胞系MCF-7、MDA-MB-231、NSCLC-N6-L16和A549以及七种人类病原菌金黄色葡萄球菌、芽孢杆菌属、蜡样芽孢杆菌、伊氏李斯特菌、大肠杆菌、弗氏柠檬酸杆菌和沙门氏菌。最显著的活性是对MCF-7细胞的细胞毒性（2 μ M）。这是首次报道在海洋真菌C. Rosea证实了其在生物活性脂质生产中的潜力。
The most common sterol in fungi is ergosterol, which has frequently been investigated in human pathogenic fungal strains. This sterol, and others isolated from fungal strains, has also demonstrated cytotoxicity against cancer cell lines and antimicrobial activities. Marine fungi can produce high amounts of bioactive compounds. So, a screening was performed to study sterol composition using GC/MS in 19 marine fungal strains and ergosterol was always the major one. One strain, Clonostachys rosea MMS1090, was selected due to its high amount of eburicol and a one strain many compounds approach was performed on seven culture media to optimize its production. After purification and structural identification by NMR, eburicol was assessed against four cancer cell lines, MCF-7, MDA-MB-231, NSCLC-N6-L16 and A549, and seven human pathogenic bacteria Staphylococcus aureus, Bacillus sp., Bacillus cereus, Listeria ivanovii, Escherichia coli, Citrobacter freundii and Salmonella spp. The most significant activity was cytotoxicity against MCF-7 cells (2 mu M). This is the first report of such an accumulation of eburicol in the marine fungal strain C. rosea confirming its potential in the production of bioactive lipids.