Growth of normal and neoplastic urothelium and response to epidermal growth factor in a defined serum-free medium.
Growth of normal and neoplastic urothelium and response to epidermal growth factor in a defined serum-free medium.
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发表时间：
1987-04
期刊：
影响因子：
11.2
通讯作者：
L. Dubeau;P. Jones
中科院分区：
文献类型：
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作者：
L. Dubeau;P. Jones
我们开发了一种确定的无血清培养基，用于正常和肿瘤性尿路上皮的体外生长。该培养基中上皮细胞的生长受到刺激，同时成纤维细胞的增殖受到抑制。该培养基允许从高级别人类膀胱癌中分离出新的细胞系。还获得了几种可以在培养物中传代数次的正常人尿路上皮菌株。尽管表皮生长因子不刺激正常和肿瘤性尿路上皮细胞在限定培养基中的生长，但50ng/ml表皮生长因子的存在诱导了提示正常尿路上皮终末成熟的形态变化，这意味着表皮生长因子要么充当上皮成熟因子，要么诱导这种因子的分泌。
We have developed a defined serum-free medium for the in vitro growth of normal and neoplastic urothelium. Growth of epithelial cells in this medium was stimulated while proliferation of fibroblasts was inhibited. The medium allowed for the isolation of a new line from a high grade human bladder carcinoma. Several strains of normal human urothelium that could be passaged several times in culture were also obtained. Although growth of normal and neoplastic urothelial cells in the defined medium was not stimulated by epidermal growth factor, the presence of 50 ng/ml epidermal growth factor induced morphological changes suggestive of terminal maturation of normal urothelium, implying that epidermal growth factor either acted as an epithelial maturation factor or induced the secretion of such a factor.