GENE-INDUCED EMBRYOLOGICAL MODIFICATIONS OF PRIMORDIAL GERM CELLS IN THE MOUSE
GENE-INDUCED EMBRYOLOGICAL MODIFICATIONS OF PRIMORDIAL GERM CELLS IN THE MOUSE
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DOI：
10.1002/jez.1401340202
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发表时间：
1957-01-01
影响因子：
--
通讯作者：
RUSSELL, ES
中科院分区：
文献类型：
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作者：
MINTZ, B;RUSSELL, ES
The embryological basis of sterility in mice of homozygous mutant W-series genotypes was studied at 8-12 days of embryonic life with the azo dye coupling technique for alkaline phosphatase staining of pri-mordial germ cells. A complete survey of numbers and locations of all germ cells was made. Germ cells of defectives form normally, at 8 days, but conspicuously fail to increase in numbers at 9 days and thereafter, and are retarded in their migration from yolk sac splanchnopleure to germinal ridges. This mitotic impairment may result indirectly from other influences on cellular metabolism. Experimental matings, employing heterozygotes, should theoretically yield 25% defective offspring. Actual frequency of embryos with very small germ cell numbers was 28-29%, statistically a good fit to expectation. In combination with later incidence of sterility, this supports the view that the stain-positive cells described are indeed the primordial germ cells, and constitutes, by the use of a genetic marker, the 1st experimental proof of the extra-gonadal origin of these cells in the mammalian embryo (Mintz and Russell, ''55). Relation to other parts of the syndrome (anemia, lack of fur pigment) is discussed in its bearing on the problem of pleiotropic gene action, and comparison with other sterility-inducing allelic series is made.
