Bacteria-induced aggregation of bioorthogonal gold nanoparticles for SERS imaging and enhanced photothermal ablation of Gram-positive bacteria
Bacteria-induced aggregation of bioorthogonal gold nanoparticles for SERS imaging and enhanced photothermal ablation of Gram-positive bacteria
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DOI：
10.1039/c9tb00845d
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发表时间：
2019-08-14
影响因子：
7
通讯作者：
Jiang, Tingting
中科院分区：
文献类型：
--
作者：
Wang, Huijie;Ouyang, Wenwen;Jiang, Tingting
抗菌光热疗法（PTT）面临的挑战是如何减少对细胞的损伤，提高抗菌效率。在此，我们报道了一种基于细菌诱导的金纳米颗粒（GNP）聚集的新治疗策略，其中GNPs通过万古霉素的特异性靶向和生物正交环加成在细菌表面原位聚集。邻近GNPs之间的等离子体耦合表现出强烈的“热点”效应，使细菌病原体的表面增强拉曼散射（SERS）成像有效。更重要的是，GNPs的原位聚集表现出强的近红外吸附和高光热转化率，从而增强了对革兰氏阳性细菌的光杀灭活性。在没有细菌菌株的情况下，GNPs分散，光热效应非常低，对周围健康组织的副作用最小。鉴于上述优点，本研究开发的生物正交治疗策略可能在治疗细菌感染甚至多重耐药细菌方面具有潜在的应用前景。
The challenge in antimicrobial photothermal therapy (PTT) is to develop strategies for decreasing the damage to cells and increasing the antibacterial efficiency. Herein, we report a novel theranostic strategy based on bacteria-induced gold nanoparticle (GNP) aggregation, in which GNPs in situ aggregated on the bacterial surface via specific targeting of vancomycin and bioorthogonal cycloaddition. Plasmonic coupling between adjacent GNPs exhibited a strong "hot spot" effect, enabling effective surface enhanced Raman scattering (SERS) imaging of bacterial pathogens. More importantly, in situ aggregation of GNPs showed strong NIR adsorption and high photothermal conversion, allowing enhanced photokilling activity against Gram-positive bacteria. In the absence of bacterial strains, GNPs were dispersed and showed a very low photothermal effect, minimizing the side effects towards surrounding healthy tissues. Given the above advantages, the bioorthogonal theranostic strategy developed in this study may find potential applications in treating bacterial infection and even multidrug-resistant bacteria.