Origin of hydrogen peroxide during the cowpea- Xanthomonas interaction
Origin of hydrogen peroxide during the cowpea- Xanthomonas interaction
复制标题
豇豆-黄单胞菌相互作用过程中过氧化氢的起源
DOI：
10.1007/s13313-011-0080-8
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发表时间：
2011-10
影响因子：
1.4
通讯作者：
Li, Hongyu
中科院分区：
文献类型：
--
作者：
Zhu, Yan;Li, Yanhong;Hu, Ronghui;Zheng, Yani;Feng, Na;Chen, Fu;Wang, Jing;Li, Hongyu
采用野生型油菜黄单胞菌（Xanthomonas campestris pv）接种豇豆幼苗，测定植物病原菌在体内产生过氧化氢（H2O2）的能力。phaseoli及其在ahpC上的突变株（H2O2产量减弱）。采用抗生素利福平（Rif）杀灭油菜黄单胞菌。豇豆叶中的菜花。Rif对豇豆组织细菌的最佳杀灭浓度为500 μg mL-1，处理时间为48 h，该浓度对豇豆叶片叶绿体和线粒体功能无显著影响。油菜黄单胞菌（Xanthomonas campestris pv）野生型和突变株接种豇豆后H2O2浓度的变化。测定Rif处理后的phaseoli。接种野生型和突变株后，豇豆叶片中H2O2积累量均有所增加。Rif处理显著降低了豇豆野生型细菌系统中过量H2O2的积累。而突变株接种后的豇豆叶片中H2O2含量与未处理的叶片相同。结果表明，体内观察到的H2O2浓度变化是由野生型细菌引起的。
To assay hydrogen peroxide (H2O2) production by phytopathogenic bacteria in vivo, cowpea seedlings were inoculated using wild-type Xanthomonas campestris pv. phaseoli and its mutant strain on ahpC (with attenuated H2O2 production). Antibiotic rifampicin (Rif) was used to kill off the Xanthomonas campestris pv. phaseoli in cowpea leaves. The optimal concentration of Rif to eradicate the bacteria was 500 μg mL–1, and duration of the Rif treatment to eradicate the bacteria in the cowpea tissues was 48 h. This level of Rif did not significantly affect chloroplast and mitochondrial function in cowpea leaves. The concentration of H2O2 in cowpea inoculated with wild-type or mutant strain of Xanthomonas campestris pv. phaseoli following Rif treatment was measured. Increased H2O2 accumulation was observed in cowpea leaves respectively inoculated with wild-type and mutant strain. The accumulation of excess H2O2 was significantly decreased by the Rif treatment on cowpea wild-type bacteria systems. However, the level of H2O2 in cowpea leaves inoculated with mutant strains following Rif treatment was at the same level as in plant leaves without Rif treatment. The results demonstrate that the change in H2O2 concentration observed in vivo was caused by wild-type bacteria.