Interactions of Saprophytic Yeasts with anor Mutant of Aspergillus flavus
Interactions of Saprophytic Yeasts with anor Mutant of Aspergillus flavus
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DOI：
10.1128/aem.65.6.2738-2740.1999
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发表时间：
1999-06
影响因子：
4.4
通讯作者：
S. Hua;J. Baker;M. Flores-Espiritu
中科院分区：
文献类型：
--
作者：
S. Hua;J. Baker;M. Flores-Espiritu
摘要黄曲霉nor突变株具有一个缺陷的去甲甲嘌呤酸还原酶，因此黄曲霉毒素的生物合成途径被阻断，导致去甲嘌呤酸（一种亮橙红色色素）的积累。我们开发了一种视觉琼脂平板测定法，通过视觉评分nor突变体的黄曲霉毒素积累来监测酵母菌株抑制黄曲霉毒素产生的能力。我们确定了酵母菌株，减少了红橙色色素积累的normutant。这些酵母菌也减少了A.黄的这些酵母可能有助于减少食品中的黄曲霉毒素污染。
ABSTRACT The nor mutant of Aspergillus flavus has a defective norsolorinic acid reductase, and thus the aflatoxin biosynthetic pathway is blocked, resulting in the accumulation of norsolorinic acid, a bright red-orange pigment. We developed a visual agar plate assay to monitor yeast strains for their ability to inhibit aflatoxin production by visually scoring the accumulation of this pigment of the nor mutant. We identified yeast strains that reduced the red-orange pigment accumulation in the normutant. These yeasts also reduced aflatoxin accumulation by a toxigenic strain of A. flavus. These yeasts may be useful for reducing aflatoxin contamination of food commodities.