Dopamine levels of two classes of vesicles are differentially depleted by amphetamine
Dopamine levels of two classes of vesicles are differentially depleted by amphetamine
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DOI：
10.1016/s0006-8993(98)00040-7
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发表时间：
1998-03-30
期刊：
影响因子：
2.9
通讯作者：
Ewing, AG
中科院分区：
文献类型：
--
作者：
Anderson, BB;Chen, GY;Ewing, AG
用安培法研究了安非他明在两类囊泡（称为大囊泡和小囊泡）中对神经递质的不同消耗。碳纤维微电极已被用于监测和量化胞吐事件。电流瞬变，对应于个人的胞吐事件，已获得从细胞体的含有多巴胺的神经元Planorbis corneus。从这些细胞的单个囊泡释放的多巴胺已经被比较了用D-安非他明处理的细胞与对照细胞。我们的研究结果表明，安非他明有不同的影响，多巴胺从两类囊泡的释放。因此，可以得出结论，在低浓度下，安非他明优先耗尽大囊泡，对小囊泡的影响最小。在高浓度下，安非他明消耗小囊泡比大囊泡更强烈，尽管安非他明继续以剂量依赖性方式消耗大囊泡。我们的数据似乎表明，在Planorbis多巴胺神经元中观察到的两类囊泡可能具有与递质耗尽相关的不同机制。(C)1998年Elsevier Science B.V.
Differential depletion of neurotransmitter by amphetamine in two classes of vesicles, termed large vesicles and small vesicles, has been studied with amperometry. Carbon fiber microelectrodes have been used to monitor and quantify exocytotic events. Current transients, corresponding to individual exocytotic events, have been obtained from the cell body of the dopamine-containing neuron of Planorbis corneus. The dopamine released from individual vesicles of these cells has been compared for cells treated with D-amphetamine vs. control cells. Our results show that amphetamine has differential effects on the release of dopamine from the two classes of vesicles. Thus, it is concluded that at low concentrations, amphetamine preferentially depletes the large vesicles with a minimal effect on the small vesicles. At high concentrations, amphetamine depletes small vesicles more strongly than large vesicles although amphetamine continues to deplete the large vesicles in a dose-dependent manner. Our data appear to indicate that the two classes of vesicles observed in the Planorbis dopamine neuron might have different mechanisms associated with transmitter depletion. (C) 1998 Elsevier Science B.V.