Fluorescence imaging of glutathione with aptasensor and monitoring deoxynivalenol-induced oxidative stress in living cells
Fluorescence imaging of glutathione with aptasensor and monitoring deoxynivalenol-induced oxidative stress in living cells
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DOI：
10.1016/j.snb.2021.131190
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发表时间：
2021-12
期刊：
影响因子：
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通讯作者：
You Zhou;Nuo Duan;Yan Lv;I. Khan;Shuo Qi;Xianfeng Lin;Wenyan Yu;Yin Zhang;Wei Xu;
中科院分区：
文献类型：
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作者：
You Zhou;Nuo Duan;Yan Lv;I. Khan;Shuo Qi;Xianfeng Lin;Wenyan Yu;Yin Zhang;Wei Xu;
谷胱甘肽（GSH）是一种重要的细胞内抗氧化剂。它与细胞内氧化还原状态的稳态密切相关，可以通过脱氧雪腐镰刀菌烯醇（DON）暴露来改变细胞内氧化还原状态的稳态。更重要的是，GSH缺乏可直接导致细胞凋亡和细胞内氧化还原稳态介导的细胞代谢紊乱。因此，迫切需要实时监测活细胞内GSH水平的动态变化来评估细胞内氧化应激。在此，首次成功构建了基于适配体的识别复合纳米探针（Apt-AuNCs-MoS2）。该纳米探针用于 DON 诱导的细胞内 GSH 变化的实时原位成像以及氧化应激状态的评估。结果表明，该适配体传感器对溶液体系中的GSH表现出优异的特异性和高灵敏度，检测限低至35 nM。有关 DON 介导的氧化应激的结果表明，当 DON 浓度超过 20 ppm 时，活细胞中的氧化应激趋于饱和。因此，该策略为GSH的测定提供了一种新型适体传感器，为活细胞内GSH的荧光成像提供了一个有前景的平台，并进一步丰富了体外毒理学的原位分析。
Glutathione (GSH) is an important intracellular antioxidant. It is closely related to the homeostasis of the intracellular redox state which can be changed through deoxynivalenol (DON) exposure. More importantly, GSH deficiency can directly lead to apoptosis and cellular metabolic disorders mediated by intracellular redox homeostasis. Therefore, real-time monitoring of dynamic changes of GSH levels in living cells is urgently needed for the evaluation of intracellular oxidative stress. Herein, for the first time, an aptamer-based recognition composite nanoprobe (Apt-AuNCs-MoS2) was successfully constructed. The nanoprobe was employed for real-time andin situimaging of DON-induced intracellular GSH changes and evaluation of oxidative stress status. The results indicated that the aptasensor revealed excellent specificity and high sensitivity to GSH in the solution system, and the limit of detection was as low as 35 nM. The results about DON-mediated oxidative stress indicated that the oxidative stress in living cells tends to be saturated with more than 20 ppm DON concentration. Thus, the strategy provides a novel aptasensor for the determination of GSH, offers a promising platform for fluorescence imaging of intracellular GSH in living cells, and further enrichesin situanalysis of thein vitrotoxicology.