Analysis of human cytomegalovirus US3 gene products.
Analysis of human cytomegalovirus US3 gene products.
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人类巨细胞病毒US3基因产物的分析。
DOI：
10.1006/viro.2002.1557
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发表时间：
2002
期刊：
影响因子：
3.7
通讯作者：
Biegalke,Bonita
中科院分区：
文献类型：
--
作者：
Liu,Wenzhong;Zhao,Yiqiang;Biegalke,Bonita
与其他疱疹病毒相似，人巨细胞病毒在原发感染消退后仍留在感染宿主体内。原发感染后在宿主中持续存在的能力被认为受到HCMV下调受感染细胞的免疫识别的能力的强烈影响。导致免疫逃避的基因之一是US3基因。US3基因已被证明在内质网中保留主要组织相容性复合体I型分子。US3基因产生三种选择性剪接的RNA，它们编码不同但相关的蛋白质。每一种可变剪接的转录本都存在于病毒感染的早期，这表明编码的蛋白质在病毒的生命周期中发挥作用。我们证明，只有由未剪接的US3转录物编码的蛋白质能够保留在内质网中的MHC I类重链。由单剪接的US3转录物编码的蛋白质似乎通过分泌途径加工，而由双剪接的转录物编码的蛋白质变得定位于高尔基体。这些实验提出了关于病毒感染宿主期间较小US3蛋白质功能的有趣问题。
Similar to other herpesviruses, human cytomegalovirus remains in the infected host following resolution of the primary infection. The ability to persist in the host after primary infection is believed to be strongly influenced by the ability of HCMV to down-regulate immune recognition of infected cells. One of the genes contributing to immune evasion is the US3 gene. The US3 gene has been shown to retain major histocompatibility complex type I molecules in the endoplasmic reticulum. The US3 gene gives rise to three alternatively spliced RNAs which encode distinct but related proteins. Each of the alternatively spliced transcripts is present early in viral infection, suggesting that the encoded proteins play a role in the viral life cycle. We demonstrate that only the protein encoded by the unspliced US3 transcript is able to retain MHC class I heavy chains in the endoplasmic reticulum. The protein encoded by the singly spliced US3 transcript appears to be processed through the secretory pathway while the protein encoded by the doubly spliced transcript becomes localized to the Golgi apparatus. These experiments raise interesting questions about the functions of the smaller US3 proteins during viral infection in the host.