Hyaluronic acid sheet transplantation attenuates infrapatellar fat pad fibrosis and pain in a rat arthritis model
Hyaluronic acid sheet transplantation attenuates infrapatellar fat pad fibrosis and pain in a rat arthritis model
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DOI：
10.1002/jor.25580
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发表时间：
2023-05-02
影响因子：
2.8
通讯作者：
Nakagawa，Yusuke
中科院分区：
文献类型：
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作者：
Qu，Zhen;Koga，Hideyuki;Nakagawa，Yusuke
髌骨下脂肪垫（IFP）纤维化发生在膝关节手术后或膝骨关节炎（KOA）期间，并引起持续疼痛和活动受限。先前的研究表明，治疗IFP纤维化可减轻动物模型的疼痛。在这项研究中，我们研究了透明质酸（HA）片移植对单碘乙酸（MIA）大鼠关节炎模型（95只雄性大鼠）IFP纤维化和关节软骨退变的影响。大鼠双侧关节内注射MIA (1.0 mg/30 μL)， 4天后手术。各组大鼠右膝移植HA片（HA组），左膝假手术（假手术组）。在注射MIA后28天的多个时间点进行失电容测试。注射后14天和28天分别进行宏观、组织学和免疫组织化学分析。采用ELISA法测定滑液中HA和白细胞介素- 1β的浓度。移植HA片可减轻注射后10 ~ 28天的持续性疼痛。透明质酸片在第14天抑制关节软骨退变。在14天和28天，IFP中的纤维化和降钙素基因相关肽阳性神经纤维末梢的侵袭均受到抑制。此外，HA片在移植后10天仍保持组织学形态。HA浓度在移植后第10天达到峰值；假手术组IL - 1β浓度在第7天显著高于HA组。因此，HA片可能是治疗KOA和膝关节手术后发生的IFP纤维化的一个有希望的选择。
Fibrosis of the infrapatellar fat pad (IFP) occurs after knee joint surgery or during knee osteoarthritis (KOA) and causes persistent pain and limited mobility. Previous studies demonstrated that treating IFP fibrosis alleviated pain in animal models. In this study, we examined the effects of hyaluronic acid (HA) sheet transplantation on IFP fibrosis and articular cartilage degeneration in a monoiodoacetic acid (MIA) rat arthritis model (95 male rats). Rats received bilateral intra‐articular MIA injections (1.0 mg/30 μL) and underwent surgery 4 days later. HA sheets were transplanted on the right knee of each rat (HA group), with the left knee receiving sham surgery (sham group). Incapacitance tests were performed at multiple time points up to 28 days after MIA injection. Macroscopic, histological, and immunohistochemical analyzes were performed 14 and 28 days after injection. The concentrations of HA and interleukin‐1β (IL‐1β) in the synovial fluid were measured using ELISA. Transplantation of HA sheets could alleviate persistent pain 10–28 days after injection. The HA sheets inhibited articular cartilage degeneration at 14 days. Fibrosis and the invasion of calcitonin gene‐related peptide‐positive nerve fiber endings in the IFP were inhibited at both 14 and 28 days. Moreover, the HA sheets remained histologically until 10 days after transplantation. The concentration of HA reached its peak on Day 10 after transplantation; the concentration of IL‐1β in the sham group was significantly higher than that in the HA group on Day 7. Therefore, HA sheets could be a promising option to treat IFP fibrosis occurring in KOA and after knee joint surgery.