Kinetics of phagocytosis and phagosome-lysosome fusion in hamster lung and peritoneal macrophages.
Kinetics of phagocytosis and phagosome-lysosome fusion in hamster lung and peritoneal macrophages.
复制标题
仓鼠肺和腹膜巨噬细胞吞噬作用和吞噬体-溶酶体融合的动力学。
DOI：
10.1002/jlb.50.3.229
复制
发表时间：
1991
影响因子：
5.5
通讯作者：
Valberg,PA
中科院分区：
文献类型：
--
作者：
Bizal,CL;Butler,JP;Feldman,HA;Valberg,PA
测定肺和腹腔巨噬细胞（LM和PM）的吞噬作用和吞噬体-溶酶体融合（PLF）的时间过程。用荧光黄标记未诱导的仓鼠LM和PM中的溶酶体。然后巨噬细胞吞噬热灭活的酿酒酵母（酵母），并在几个时间点评估酵母颗粒粘附与内化的程度，以及基于含酵母吞噬体中是否存在荧光黄，是否存在PLF。开发了独立吞噬作用和PLF的三室模型（粘附，摄入，融合）;对每个隔室中的酵母颗粒的数量进行计数，并确定摄入和融合的速率常数。速率常数的比较显示，PM的摄食（0.047 ± 0.005 min-1）显著快于LM（0.016 ± 0.005 min-1）（平均值±合并SEM; P <0.001）。PMs的PLF（0.109 ± 0.013 min-1）显著快于LMs（0.046 ± 0.013 min-1）（P <0.003）。
The time course of phagocytosis and phagosome-lysosome fusion (PLF) in lung and peritoneal macrophages (LMs and PMs) was measured. Lysosomes in unelicited hamster LMs and PMs were labeled with lucifer yellow. Macrophages then phagocytized heat-killedSaccharomyces cerevisiae(yeast) and were evaluated at several time points for the degree to which yeast particles were adherent vs. internalized and for the presence or absence of PLF as based on the presence or absence of lucifer yellow in yeast-containing phagosomes. A three-compartment model (adherent, ingested, fused) of independent phagocytosis and PLF was developed; the number of yeast particles in each compartment was counted, and rate constants for ingestion and fusion were determined. Comparison of rate constants showed that ingestion was significantly faster in PMs (0.047 ± 0.005 min-1) than in LMs (0.016 ± 0.005 min-1) (mean ± pooled SEM;P< 0.001). Similarly, PLF was significantly faster in PMs (0.109 ± 0.013 min-1) than in LMs (0.046 ± 0.013 min-1) (P< 0.003).