Preparation and hydration control of corneal tissue strips for experimental use
Preparation and hydration control of corneal tissue strips for experimental use
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DOI：
10.1097/00003226-200401000-00010
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发表时间：
2004-01-01
期刊：
影响因子：
2.8
通讯作者：
Parel, JM
中科院分区：
文献类型：
--
作者：
Borja, D;Manns, F;Parel, JM
目的：建立一种在角膜生物力学和热稳定性实验研究中保持角膜组织条正常水化状态，避免角膜组织肿胀或脱水的实验方案。方法：将9只全眼库眼球浸泡在25%葡聚糖溶液中24小时，使角膜厚度恢复到正常值。然后取下角膜纽扣，切成2×6 mm的条状。将9条试条依次浸入浓度为25%、22.5%和20%的葡聚糖溶液中，温度为35℃。结果：2 5%、2 2.5%、2 0%葡聚糖溶液浸泡1h后，9片角膜片的平均厚度分别为5 0 8 38、5 63 5 6、5 92 33 pm。在25%、22.5%和20%葡聚糖溶液中的平均溶胀分别为初始厚度的1.06+/-0.03、1.20+/-0.07和1.24+/-0.07倍。结论：当角膜组织条浸泡在25%葡聚糖溶液中时，角膜组织条的水合作用保持在正常水平。在分子量较低的溶液中，角膜条会膨胀到高于正常值的厚度。
Purpose: The purpose of this study was to develop an experimental protocol to maintain corneal tissue strips at normal hydration and avoid swelling or dehydration during experimental studies on corneal biomechanics and thermal stability.Methods: The corneal thickness of 9 whole Eye-Bank eyes was brought back to normal values by immersion in a 25% dextran solution for 24 hours. Corneal buttons were then removed and cut into 2 x 6 mm strips. The 9 strips were immersed successively in Dextran solutions at 25%, 22.5%, and 20% concentration at 35degreesC. The thickness of the immersed strip in solution was measured every 5 minutes for 1 hour using a modified optical comparator.Results: The mean final thickness for the nine corneal strips after one hour in 25%, 22.5%, and 20% dextran solution was 508 38, 563 56, and 592 33 pm, respectively. Average swelling in 25%, 22.5%, and 20% dextran solutions was 1.06 +/- 0.03, 1.20 +/- 0.07, and 1.24 +/- 0.07 times the initial thickness, respectively.Conclusion: The hydration of corneal tissue strips is maintained at normal values when the strips are immersed in 25% Dextran solution. Corneal strips swell to thicknesses above normal values in solutions with lower molecular weights.