Maternal inheritance of bifidobacterial communities and bifidophages in infants through vertical transmission.
Maternal inheritance of bifidobacterial communities and bifidophages in infants through vertical transmission.
复制标题
DOI：
10.1186/s40168-017-0282-6
复制
发表时间：
2017-06-26
期刊：
影响因子：
15.5
通讯作者：
Ventura M
中科院分区：
文献类型：
--
作者：
Duranti S;Lugli GA;Mancabelli L;Armanini F;Turroni F;James K;Ferretti P;Gorfer V;Ferrario C;Milani C;Mangifesta M;Anzalone R;Zolfo M;Viappiani A;Pasolli E;Bariletti I;Canto R;Clementi R;Cologna M;Crifò T;Cusumano G;Fedi S;Gottardi S;Innamorati C;Masè C;Postai D;Savoi D;Soffiati M;Tateo S;Pedrotti A;Segata N;van Sinderen D;Ventura M
人类肠道微生物群的正确建立代表了从出生开始的关键发展。不同的假设提出，婴儿肠道微生物群来源于母亲的粪便/阴道微生物群和母乳等。基于内转录间隔区（ITS）的方法，结合培养介导的和基因组分析的25个母婴对的双歧杆菌群落的组成进行了研究。我们鉴定了母亲和其相应新生儿之间共享的双歧杆菌菌株/群落。值得注意的是，基因组分析和生长谱测定显示，从人乳中分离的双歧杆菌菌株在遗传上适应利用人乳聚糖。此外，我们还鉴定出了特定的噬菌体，这些噬菌体特异于在母亲和相应的孩子的病毒组中常见的双歧杆菌属细菌。这项研究强调了双歧杆菌群落从母亲到她的孩子的传播，并暗示母乳是促进这种收购的潜在载体。此外，这些数据代表了母亲遗传双歧杆菌的第一个例子，也被称为婴儿中的双歧噬菌体，通过垂直传播途径。本文的在线版本（doi：10.1186/s40168-017-0282-6）包含补充材料，可供授权用户使用。
The correct establishment of the human gut microbiota represents a crucial development that commences at birth. Different hypotheses propose that the infant gut microbiota is derived from, among other sources, the mother’s fecal/vaginal microbiota and human milk. The composition of bifidobacterial communities of 25 mother-infant pairs was investigated based on an internal transcribed spacer (ITS) approach, combined with cultivation-mediated and genomic analyses. We identified bifidobacterial strains/communities that are shared between mothers and their corresponding newborns. Notably, genomic analyses together with growth profiling assays revealed that bifidobacterial strains that had been isolated from human milk are genetically adapted to utilize human milk glycans. In addition, we identified particular bacteriophages specific of bifidobacterial species that are common in the viromes of mother and corresponding child. This study highlights the transmission of bifidobacterial communities from the mother to her child and implies human milk as a potential vehicle to facilitate this acquisition. Furthermore, these data represent the first example of maternal inheritance of bifidobacterial phages, also known as bifidophages in infants following a vertical transmission route. The online version of this article (doi:10.1186/s40168-017-0282-6) contains supplementary material, which is available to authorized users.