Single, High-Dose Local Injection of bFGF Improves Thyroarytenoid Muscle Atrophy After Paralysis
Single, High-Dose Local Injection of bFGF Improves Thyroarytenoid Muscle Atrophy After Paralysis
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DOI：
10.1002/lary.27887
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发表时间：
2020-01-01
期刊：
影响因子：
2.6
通讯作者：
Yamasoba, Tatsuya
中科院分区：
文献类型：
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作者：
Goto, Takao;Ueha, Rumi;Yamasoba, Tatsuya
目的/假设：单侧声带麻痹(UVFP)是由于失神经甲状旁肌(TA)进行性萎缩所致的声音嘶哑。因此，需要对萎缩的TA肌进行再生治疗。碱性成纤维细胞生长因子参与肌肉的发育和再生。研究设计：动物研究。方法：建立大鼠喉返神经麻痹模型，28d后于TA肌内注射低剂量(200 Ng)或大剂量(2000 Ng)bFGF或生理盐水(对照组)。分别于治疗后第1、3、7、14、28天摘除喉。比较正常侧和瘫痪侧TA肌横截面积，计数Ki67阳性分裂细胞(Ki67(+))、配对Box 7(Pax7(+))卫星细胞(SCs)和成肌样分化阳性细胞(MyoD(+))。结果：大剂量碱性成纤维细胞生长因子治疗28d后，TA肌面积随时间延长而增大，与生理盐水对照组比较差异有统计学意义(P
Objectives/Hypothesis: Unilateral vocal fold paralysis (UVFP) induces hoarseness due to progressive atrophy of the denervated thyroarytenoid (TA) muscle. Therefore, treatments aimed at regenerating the atrophied TA muscle are required. Basic fibroblast growth factor (bFGF) is involved in muscle development and regeneration. This study aimed to elucidate the effects of bFGF injection on atrophied TA muscle.Study Design: Animal research.Methods: A recurrent laryngeal nerve-paralysis rat model was established, and low- (200 ng) or high-dose (2,000 ng) bFGF or saline (control) was injected into the TA muscle 28 days later. The larynges were excised on day 1, 3, 7, 14, and 28 after treatment. The cross-sectional area of the TA muscle in normal and paralyzed sides was compared, and the Ki67-positive (Ki67(+)) dividing cells, paired box 7-positive (Pax7(+)) satellite cells (SCs), and myogenic differentiation-positive (MyoD(+)) myoblasts were counted.Results: The TA muscle area of animals administered high-dose bFGF increased with time and was significantly larger than that of the saline-injected controls 28 days after treatment (P