A cellular and regulatory map of the GABAergic nervous system of C. elegans
A cellular and regulatory map of the GABAergic nervous system of C. elegans
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DOI：
10.7554/elife.17686
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发表时间：
2016-10-14
期刊：
影响因子：
7.7
通讯作者：
Hobert, Oliver
中科院分区：
文献类型：
--
作者：
Gendrel, Marie;Atlas, Emily G.;Hobert, Oliver
神经递质图是解剖图的重要补充，是了解神经系统功能和发育的宝贵资源。我们在此报告了线虫神经系统中含有神经递质 GABA 的神经元的综合图谱，显示的 GABA 阳性神经元类别数量是之前报道的两倍。我们定义了以前未知的摄取 GABA 的神经胶质样细胞，以及不合成 GABA 而是从细胞外环境中摄取 GABA 的“GABA 摄取神经元”，并且我们绘制了以前未表征的离子型 GABA 受体的表达图。我们使用 GABA 阳性神经元图谱对定义 GABA 表型的转录调节因子进行全面分析。我们将 GABA 能神经元规范的发现与之前关于谷氨酸能和胆碱能神经元规范的报告综合成一个神经系统范围的调节图，该图定义了线虫中一半以上所有神经元类别的神经递质规范机制。
Neurotransmitter maps are important complements to anatomical maps and represent an invaluable resource to understand nervous system function and development. We report here a comprehensive map of neurons in the C. elegans nervous system that contain the neurotransmitter GABA, revealing twice as many GABA-positive neuron classes as previously reported. We define previously unknown glia-like cells that take up GABA, as well as 'GABA uptake neurons' which do not synthesize GABA but take it up from the extracellular environment, and we map the expression of previously uncharacterized ionotropic GABA receptors. We use the map of GABA-positive neurons for a comprehensive analysis of transcriptional regulators that define the GABA phenotype. We synthesize our findings of specification of GABAergic neurons with previous reports on the specification of glutamatergic and cholinergic neurons into a nervous system-wide regulatory map which defines neurotransmitter specification mechanisms for more than half of all neuron classes in C. elegans.