Mapping the Escherichia coli transcription elongation complex with exonuclease III.
Mapping the Escherichia coli transcription elongation complex with exonuclease III.
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使用核酸外切酶 III 绘制大肠杆菌转录延伸复合物图谱。
DOI：
10.1007/978-1-4939-2392-2_1
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发表时间：
2015
期刊：
影响因子：
--
通讯作者：
Artsimovitch,Irina
中科院分区：
文献类型：
--
作者：
Liu,Zhaokun;Artsimovitch,Irina
RNA polymerase interactions with the nucleic acids control every step of the transcription cycle. These contacts mediate RNA polymerase recruitment to promoters, induce pausing during RNA chain synthesis, and control transcription termination. These interactions are dissected using footprinting assays, in which a bound protein protects nucleic acids from the digestion by nucleases or modification by chemical probes. Exonuclease III is frequently employed to study protein–DNA interactions owing to relatively simple procedures and low background. Exonuclease III has been used to determine RNA polymerase position in transcription initiation and elongation complexes and to infer the translocation register of the enzyme. In this chapter, we describe probing the location and the conformation of transcription elongation complexes formed by walking of the RNA polymerase along an immobilized template.
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影响因子：
16
作者：
Belogurov, Georgiy A.;Vassylyeva, Marina N.;Artsimovitch, Irina
通讯作者：
Artsimovitch, Irina
影响因子：
14.9
作者：
Zhilina E;Esyunina D;Brodolin K;Kulbachinskiy A
通讯作者：
Kulbachinskiy A
影响因子：
16
作者：
Temiakov, D;Zenkin, N;Vassylyev, DG
通讯作者：
Vassylyev, DG
影响因子：
4.8
作者：
Spitalny, P;Thomm, M
通讯作者：
Thomm, M
用核酸外切酶 III 进行足迹分析。
影响因子：
--
作者：
W. Metzger;H. Heumann
通讯作者：
H. Heumann