Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins
Effects of plant hormones and shading on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins
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DOI：
10.1016/j.plantsci.2004.03.021
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发表时间：
2004-08-01
期刊：
影响因子：
5.2
通讯作者：
Esaka, A
中科院分区：
文献类型：
--
作者：
Jeong, ST;Goto-Yamamoto, N;Esaka, A
花青素是葡萄酒酿造中重要的葡萄成分。本文研究了植物激素、脱落酸（ABA）和萘乙酸（NAA）以及遮光处理对葡萄果皮花青素积累和花青素生物合成基因表达的影响。ABA处理促进了花青素的积累，NAA和遮荫抑制了花青素的积累。ABA增强了葡萄花青素生物合成调控基因VwnybAl和所有花青素生物合成途径酶基因的mRNA积累，而NAA和遮光则抑制和延缓了这些基因的积累。VvmybAl mRNA拷贝数的变化与所有被测花青素生物合成酶基因的变化和花青素积累的变化一致。我们还发现，在葡萄浆果皮着色过程中，三个Chss之间、两个Chis之间和两个F3hs之间的mRNA拷贝数存在显著差异。2004爱思唯尔爱尔兰有限公司版权所有。
Anthocyanins are important grape components for red wine making. Here we examined the effects of plant hormones, abscisic acid (ABA) and naphthaleneacetic acid (NAA), and shading treatments on the accumulation of anthocyanins and the expression of anthocyanin biosynthetic genes in grape berry skins. The accumulation of anthocyanins was enhanced by ABA treatment and suppressed by NAA and shading. ABA enhanced the mRNA accumulation of VwnybAl, a putative regulatory gene of anthocyanin biosynthesis of grape, and all the tested enzyme genes of the anthocyanin biosynthetic pathway, while NAA and shading suppressed and retarded them. The change of the mRNA copy number of VvmybAl coincided with that of all the tested anthocyanin biosynthetic enzyme genes and the accumulation of anthocyanins. We also showed that there were significant differences in the mRNA copy number among three Chss, between two Chis, and two F3hs during the coloration of grape berry skins. (C) 2004 Elsevier Ireland Ltd. All rights reserved.