The modified HET-CAM as a model for the assessment of the inflammatory response to tissue tolerable plasma
The modified HET-CAM as a model for the assessment of the inflammatory response to tissue tolerable plasma
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DOI：
10.1016/j.tiv.2010.11.012
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发表时间：
2011-03-01
影响因子：
3.2
通讯作者：
Huebner, Nils-Olaf
中科院分区：
文献类型：
--
作者：
Bender, Claudia;Partecke, Lars-Ivo;Huebner, Nils-Olaf
血浆医学是一个新的、高度交叉的研究领域。虽然关于基于血浆的生物医学机制、正确的剂量和治疗次数的知识很少，但已经进行了动物实验。为了遵循3Rs(缩减、精化、替换)，有必要定义血浆参数的筛选方法。为了确定可靠的测试方法并验证组织耐受血浆(UP)用于慢性创面治疗的有效性，我们选择了改良的鸡卵绒毛尿囊膜试验(MOD)。HET-CAM)作为衡量血浆炎症潜能的模型。使用HF-等离子体射流引起的不同强度的炎症与等离子体-组织相互作用的时间相对应。此外，改变了等离子体模式和气体成分，以评估它们对治疗效果的影响。脉冲等离子体导致最轻微的炎症，而向Ar载气中添加0.1%的氧气导致最明显的反应。结果表明，曝光时间的影响大于模式和气体成分的影响。当血浆治疗后立即加入氢化可的松(HC)时，所有炎症反应都得到缓解。研究结果表明，改良的HET-CAM试验适用于血浆来源的筛选和确定治疗慢性创面的最佳参数。为了将慢性创面转移到没有有害炎症的急性愈合创面，血浆-组织相互作用的最长时间不应超过5 S与受试血浆来源。此外，有可能在绒毛尿囊膜上诱导明确的血浆炎症反应，并测定受试物质的抗炎潜力。(C)2010爱思唯尔有限公司。保留所有权利。
Plasma medicine is a novel, highly interdisciplinary field of research. Although the knowledge is rare concerning plasma based biomedical mechanisms, correct dosages and treatment times, animal experiments have been carried out. To follow the 3Rs (reduction, refinement, replacement), it is necessary to define methods for the screening of plasma parameters.In order to determine a reliable test and validate the use of tissue tolerable plasma (UP) for the treatment of chronic wounds, we have selected the modified hen's egg test on the chorioallantoic membrane (mod. HET-CAM) as a model to benchmark the inflammation potential of plasma.Inflammations of different intensities provoked by using an HF-plasma jet corresponded to the time of plasma-tissue interaction. Additionally, the plasma mode and the gas composition were changed to assess their influence on the efficacy of treatment. Pulsed plasma led to the mildest inflammation, while the addition of 0.1% oxygen to the argon carrier gas led to the most distinct reaction. It was found that the influence of the exposure time was greater than that of the mode and the gas composition. All inflammations were alleviated, when hydrocortisone (HC) was added immediately after plasma treatment.The results of the study demonstrate that the modified HET-CAM test is suitable for screening plasma sources and for the determination of the optimum parameters for treatment of chronic wounds. To transfer the chronic wound into an acute healing wound without harmful inflammation, the maximum time for plasma-tissue interaction should not exceed 5 s with the tested plasma source.Furthermore, it is possible to induce defined plasma-inflammations on the chorioallantoic membrane and to determine the anti-inflammatory potential of test substances. (C) 2010 Elsevier Ltd. All rights reserved.