TRANS-NEURONAL RETROGRADE DEGENERATION OF RETINAL GANGLION-CELLS AFTER DAMAGE TO STRIATE CORTEX IN MACAQUE MONKEYS - SELECTIVE LOSS OF P-BETA CELLS
TRANS-NEURONAL RETROGRADE DEGENERATION OF RETINAL GANGLION-CELLS AFTER DAMAGE TO STRIATE CORTEX IN MACAQUE MONKEYS - SELECTIVE LOSS OF P-BETA CELLS
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DOI：
10.1016/0306-4522(89)90333-3
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发表时间：
1989-01-01
期刊：
影响因子：
3.3
通讯作者：
PERRY, VH
中科院分区：
文献类型：
--
作者：
COWEY, A;STOERIG, P;PERRY, VH
我们检查了两只8年前左侧纹状皮质被切除的猴子的视网膜，并将每只眼睛跨神经变性的半视网膜与正常半视网膜以及正常猴子的视网膜进行了比较。所有的视网膜都准备成完整的支架。每对中的一对进行甲酚紫染色，另一对在将辣根过氧化物酶小球放入视神经两天后进行辣根过氧化物酶反应。对侧右眼视网膜尼氏染色的神经节细胞密度测量显示，约80%的视网膜神经节细胞在中央30度处缺失。退化的半视网膜。在更小的范围内，百分比损失没有那么广泛。对同侧变性的颞侧视网膜中经过氧化物酶标记的存活细胞的胞体大小和树突野大小的测量表明，它们在形态上是正常的。然而，与正常的半视网膜相比，在三个选定的偏心处的平均胞体大小比正常大，这表明选择性地丢失了较小的神经节细胞。对正常侧视网膜和变性侧视网膜内的神经节细胞进行了分类，发现P.beta的种群。变性区域的细胞减少了高达85%。P.pha的密度没有相应的变化。或者P.Gamma。细胞。绝大多数P.beta的退化。细胞被认为是神经节细胞的形态底物，具有较小的与颜色相反的感受野，必须限制纹状皮质损伤后幸存下来的视觉敏感性和辨别力。
We examined the retinae of two monkeys whose left striate cortex had been removed eight years previously and compared the transneuronally degenerated hemiretina of each eye with the normal hemiretina, and with the retinae of normal monkeys. All retinae were prepared as whole mounts. One from each pair was stained with Cresyl Violet; the other was reacted for horseradish peroxidase two days after placing pellets of the enzyme in the optic nerve. Measurements of ganglion cell density in the Nissl-stained retina of the contralateral right eye showed that approximately 80% of retinal ganglion cells were missing in the central 30.degree. of the degenerated hemiretinae. More peripherally the percentage loss was less extensive. Measurements of cell soma size and dendritic field size of peroxidase-labelled classified surviving cells in the degenerated temporal hemiretina of the ipsilateral eye showed them to be morphologically normal. In comparison with the normal hemiretina, however, the mean soma size at three selected eccentricities was larger than normal, suggesting selective loss of smaller ganglion cells. Classification of peroxidase-labelled ganglion cells in the normal and degenerated hemiretinae revealed that the population of P.beta. cells was reduced by as much as 85% in the degenerated region. There was no comparable change in the density of P.alpha. or P.gamma. cells. The degeneration of the great majority of P.beta. cells, which are believed to be the morphological substrate of ganglion cells with small and color-opponent receptive fields, must set limits on the visual sensitivity and discrimination that survive damage to striate cortex.