Positive/negative surface charge of chitosan based nanogels and its potential influence on oral insulin delivery
Positive/negative surface charge of chitosan based nanogels and its potential influence on oral insulin delivery
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DOI：
10.1016/j.carbpol.2015.09.103
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发表时间：
2016-01-20
影响因子：
11.2
通讯作者：
Chen, Xiguang
中科院分区：
文献类型：
--
作者：
Wang, Juan;Xu, Mengxue;Chen, Xiguang
为开发治疗糖尿病的胰岛素缓释系统，制备了两种Zeta电位相反的胰岛素纳米凝胶(-15.94+/-0.449 mV胰岛素：CMCS/CS-NGS(-)和+17.15+/-0.492 mV：CMCS/CS-NGS(+))。体外实验结果表明，表面电荷相反的纳米凝胶在特定的肠段具有不同的粘附性和渗透性。胰岛素：CMCS/CSNGs(-)在大鼠十二指肠的粘附性和渗透性无明显差异，但在大鼠空肠中表现出明显的粘附性和渗透性，分别是胰岛素：CMCS/CS-NGS(+)的3倍和1.7倍。这些结果表明，纳米凝胶的表面电荷性质决定了CMCS/CS-NGS在小肠中的吸收位置。在体内实验中，胰岛素：CMCS/CS-NGS(-)组在给药后1~11h的血糖水平比胰岛素：CMCS/CSNGS(+)组低3 mmol/L，说明阴性胰岛素：CMCS/CS-NGS比阳性胰岛素：CMCS/CS-NGS能更好地控制血糖。(C)2015爱思唯尔有限公司。保留所有权利。
To develop insulin delivery system for the treatment of diabetes, two insulin-loaded nanogels with opposite zeta potential (-15.94 +/- 0.449 mV for insulin:CMCS/CS-NGs(-) and +17.15 +/- 0.492 mV for insulin:CMCS/CS-NGs(+)) were obtained. Ex vivo results showed that the nanogels with opposite surface charge exhibited different adhesion and permeation in specific intestinal segments. There was no significant differences in adhesion and permeation in rat duodenum, but in rat jejunum, insulin: CMCS/CSNGs(-) exhibited enhanced adhesion and permeation, which were about 3 folds (adhesion) and 1.7 folds (permeation) higher than insulin: CMCS/CS-NGs(+). These results demonstrated that the surface charge property of nanogels determined the absorption sites of CMCS/CS-NGs in small intestine. In vivo study, the blood glucose level in insulin:CMCS/CS-NGs(-) group had 3 mmol/L lower than insulin:CMCS/CSNGs(+) group during 1 h to 11 h after the oral administration, which demonstrated that negative insulin: CMCS/CS-NGs had a better management of blood glucose than positive ones. (C) 2015 Elsevier Ltd. All rights reserved.