Novel biosensing methodologies for improving the detection of single nucleotide polymorphism
Novel biosensing methodologies for improving the detection of single nucleotide polymorphism
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改进单核苷酸多态性检测的新型生物传感方法
DOI：
10.1016/j.bios.2014.11.041
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发表时间：
2015-04-15
影响因子：
12.6
通讯作者：
Chen, Ming
中科院分区：
文献类型：
--
作者：
Chang, Kai;Deng, Shaoli;Chen, Ming
越来越多的序列数据证实了越来越多的候选单核苷酸多态性（SNP），这被认为是揭示个体对疾病的易感性和对治疗的不同反应的遗传基础。因此，迫切需要开发灵敏、快速、易于使用和成本有效的方法来鉴定SNPs。在过去的二十年中，生物传感技术已经发展，通过整合生物反应的独特特异性和物理传感器的高灵敏度，这为SNP的检测提供了显着的优势。在这篇专题文章中，我们重点介绍了基于生物传感器的SNP基因分型策略，包括核酸类似物、表面连接反应、单碱基延伸、错配结合蛋白、分子信标、滚环扩增和链置换扩增。此外，还讨论了它们的优点、目前的局限性和未来的发展趋势。生物传感技术将为SNPs的检测提供一种有前途的替代方法，并为遗传疾病的诊断和设计适当的治疗方法铺平道路。(C)© 2014 Elsevier B.V.保留所有权利。
The growing volume of sequence data confirm more and more candidate single nucleotide polymorphisms (SNPs), which are believed to reveal the genetic basis of individual susceptibility to disease and the diverse responses to treatment. There is therefore an urgent demand for developing the sensitive, rapid, easy-to-use, and cost-effective method to identify SNPs. During the last two decades, biosensing techniques have been developed by integrating the unique specificity of biological reactions and the high sensitivity of physical sensors, which provided significant advantages for the detection of SNPs. In this feature article, we focused attention on the strategies of SNP genotyping based on biosensors, including nucleic acid analogs, surface ligation reaction, single base extension, mismatch binding protein, molecular beacon, rolling circle amplification, and strand-displacement amplification. In addition, the perspectives on their advantages, current limitations, and future trends were also discussed. The biosensing technique would provide a promising alternative for the detection of SNPs, and pave the way for the diagnosis of genetic diseases and the design of appropriate treatments. (C) 2014 Elsevier B.V. All rights reserved.