Naive recirculating B cells mature simultaneously in the spleen and bone marrow
Naive recirculating B cells mature simultaneously in the spleen and bone marrow
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DOI：
10.1182/blood-2006-05-021089
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发表时间：
2007-03-15
期刊：
影响因子：
20.3
通讯作者：
Pillai, Shiv
中科院分区：
文献类型：
--
作者：
Cariappa, Annaiah;Chase, Catharine;Pillai, Shiv
我们最近证明，除了在传统的次级淋巴器官中建立良好的滤泡壁龛外，IgD(hi) B细胞还可以占据骨髓血管外的窦周壁龛。长期以来，脾脏一直被认为是新形成的B淋巴细胞成熟为IgD（hi）幼稚再循环B细胞的部位，但在骨髓中选择性丢失成熟B细胞的突变小鼠的存在促使了对后一部位B细胞成熟的检查。在完整小鼠中进行一次BrdU脉冲后，对骨髓中更成熟的b细胞群进行顺序标记，表明该部位正在进行成熟。骨髓中B细胞成熟的进一步证据来自对脾脏切除的淋巴素α缺陷小鼠的移行B细胞的分析，这些小鼠缺乏所有的次级淋巴器官。在这些小鼠中，在静脉微生物攻击后，在骨髓中也观察到识别多价抗原的抗体分泌细胞。这些数据表明，新形成的B细胞在脾脏和骨髓中同时成熟为IgD(hi) B细胞，并以严格的方式建立了体液免疫反应可以在骨髓中原位启动。
We have recently demonstrated that IgD(hi) B cells can occupy an extravascular perisinusoidal niche in the bone marrow in addition to the well-established follicular niche in conventional secondary lymphoid organs. The spleen has long been considered to be the site at which newly formed B lymphocytes mature into IgD(hi) naive recirculating B cells, but the existence of mutant mice that have selectively lost mature B cells in the bone marrow prompted an examination of B-cell maturation at this latter site. Following a single pulse of BrdU in intact mice, sequential labeling of more mature B-cell populations in the bone marrow suggested ongoing maturation at this site. Further evidence for B-cell maturation in the bone marrow was obtained from analyses of transitional B cells in splenectomized lymphotoxin alpha-deficient mice that lack all secondary lymphold organs. In these mice, antibody-secreting cells recognizing multivalent antigens were also observed in the bone marrow following an intravenous microbial challenge. These data suggest that newly formed B cells mature into IgD(hi) B cells simultaneously in the spleen and the bone marrow and establish in a stringent manner that humoral immune responses can be initiated in situ in the bone marrow.