Development of an Aerosol Surface Inoculation Method for Bacillus Spores
Development of an Aerosol Surface Inoculation Method for Bacillus Spores
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芽孢杆菌孢子气溶胶表面接种方法的开发
DOI：
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发表时间：
2010
影响因子：
4.4
通讯作者：
E. Snyder
中科院分区：
文献类型：
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作者：
Sang Don D Lee;S. Ryan;E. Snyder
摘要本研究发展了一种将枯草芽孢杆菌存款于不同表面材料上的方法，用于生物制剂的去污和检测研究。这种新方法使用与定量吸入器耦合的装置，以可重复地将孢子存款到各种表面上。定量吸入器装有枯草芽孢杆菌孢子，一种炭疽芽孢杆菌的替代物。五种不同的材料表面（铝，镀锌钢，木材，地毯，和油漆滤纸）进行了测试，使用这种孢子沉积方法。该雾化方法沉积的孢子浓度超过107 CFU/试样（直径18 mm），变异系数小于50%，表明本研究中开发的雾化方法可以将可重现数量的孢子存款到各种表面试样上。扫描电子显微镜用于探测测试试样上的孢子沉积模式。将气溶胶撞击后观察到的沉积模式与液体接种进行比较。观察到两种不同方法导致孢子沉积模式的物理差异。本研究中开发的孢子沉积方法将有助于通过雾化快速且可重复地制备孢子试样，用于台架去污和检测研究。
ABSTRACT A method was developed to deposit Bacillus subtilis spores via aerosolization onto various surface materials for biological agent decontamination and detection studies. This new method uses an apparatus coupled with a metered dose inhaler to reproducibly deposit spores onto various surfaces. A metered dose inhaler was loaded with Bacillus subtilis spores, a surrogate for Bacillus anthracis. Five different material surfaces (aluminum, galvanized steel, wood, carpet, and painted wallboard paper) were tested using this spore deposition method. This aerosolization method deposited spores at a concentration of more than 107 CFU per coupon (18-mm diameter) with less than a 50% coefficient of variation, showing that the aerosolization method developed in this study can deposit reproducible numbers of spores onto various surface coupons. Scanning electron microscopy was used to probe the spore deposition patterns on test coupons. The deposition patterns observed following aerosol impaction were compared to those of liquid inoculation. A physical difference in the spore deposition patterns was observed to result from the two different methods. The spore deposition method developed in this study will help prepare spore coupons via aerosolization fast and reproducibly for bench top decontamination and detection studies.