A scalable membrane gradostat reactor for enzyme production using Phanerochaete chrysosporium
A scalable membrane gradostat reactor for enzyme production using Phanerochaete chrysosporium
复制标题
使用黄孢原毛平革菌 (Phanerochaete chrysosporium) 生产酶的可扩展膜梯度反应器
DOI：
10.1023/a:1021963201340
复制
发表时间：
2004
影响因子：
2.7
通讯作者：
V. L. Pillay
中科院分区：
文献类型：
--
作者：
S. Govender;E. Jacobs;W. Leukes;V. L. Pillay
这是利用黄孢平革菌me446连续生产酶的膜渐变反应器的第一次工艺放大演示。通过反过滤将真菌固定在外部未剥皮的超滤毛细管膜上，然后在生物膜上诱导营养梯度。将单毛细管生物反应器的10倍放大到2.4 l多毛细管反应器，酶产量增加了7倍，峰值为209 U l−1d−1。讨论了后续规模效应对孢子分布、锰过氧化物酶连续生产剖面和生物膜发育的影响。
This is the first demonstration of process scale-up of a membrane gradostat reactor for continuous enzyme production usingPhanerochaete chrysosporiumME446. The fungus was immobilised by reverse filtration on to externally unskinned, ultrafiltration capillary membranes and then nutrient gradients were induced across the biofilm. A 10-fold scale-up from a single capillary bioreactor to a 2.4 l multi-capillary unit resulted in a 7-fold increase in enzyme productivity with a peak at 209 U l−1d−1. Subsequent scale effects on the spore distribution, continuous manganese peroxidase production profile and biofilm development are discussed.