Concentration-dependent substate behavior of native AMPA receptors.
Concentration-dependent substate behavior of native AMPA receptors.
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天然 AMPA 受体的浓度依赖性亚状态行为。
DOI：
10.1038/79931
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Howe,JR
中科院分区：
文献类型：
--
作者：
Smith,TC;Howe,JR
AMPA型谷氨酸受体介导中枢突触的大多数兴奋性突触后电流（EPSC），其电导部分决定了EPSC的大小。重组AMPA受体的电导取决于与通道结合的激动剂分子的数量。在这里，我们测试是否本地AMPA和红藻氨酸受体显示这种行为在外向补丁从原位神经元通过测量电导水平的单通道在很宽的范围内的激动剂浓度。我们发现，AMPA，但不是红藻氨酸，受体的电导强烈依赖于激动剂浓度。我们的研究结果表明，在突触间隙中的谷氨酸浓度的改变可能会改变突触后AMPA受体的表观单一电导。
AMPA-type glutamate receptors mediate most excitatory postsynaptic currents (EPSCs) at central synapses, and their conductance determines in part the size of EPSCs. The conductance of a recombinant AMPA receptor depends on the number of agonist molecules bound to the channel. Here we tested whether native AMPA and kainate receptors show this behavior in outside-out patches from neurons in situ by measuring conductance levels of single channels over a wide range of agonist concentrations. We found that the conductance of AMPA, but not kainate, receptors depended strongly on agonist concentration. Our results suggest that alterations in the glutamate concentration in the synaptic cleft may change the apparent unitary conductance of postsynaptic AMPA receptors.