Detection of Neural Action Potentials Using Optical Coherence Tomography: Intensity and Phase Measurements with and without Dyes.
Detection of Neural Action Potentials Using Optical Coherence Tomography: Intensity and Phase Measurements with and without Dyes.
复制标题
DOI：
10.3389/fnene.2010.00022
复制
发表时间：
2010-01-01
期刊：
影响因子：
--
通讯作者：
Sivaprakasam, Aarthi
中科院分区：
文献类型：
--
作者：
Akkin, Taner;Landowne, David;Sivaprakasam, Aarthi
我们回顾了使用光学相干断层扫描（OCT）检测神经活动，并提出了一种新的方法，深度定位的神经动作电位（AP），使用电压敏感染料作为对比剂在OCT。染色鱿鱼巨大的轴突成像的光谱域OCT。在AP传播过程中测量来自膜周围区域的背散射光的强度和相位的变化。后向散射强度的深度分辨变化与AP到达测量区域一致，并且与独立测量的透射光强度和反射模式交叉偏振光强度的变化同步。该系统还提供深度分辨相位变化作为活动的附加指示。随着进一步的研究，我们的结果可能会打开一个新时代的功能成像技术定位在不同深度的神经活动在原位。
We review the use of optical coherence tomography (OCT) for detection of neural activity, and present a new approach for depth-localization of neural action potentials (APs) using voltage-sensitive dyes as contrast agents in OCT. A stained squid giant axon is imaged by spectral-domain OCT. Changes in the intensity and phase of back-scattered light coming from regions around the membrane are measured during AP propagation. The depth-resolved change in back-scattered intensity coincides with the arrival of AP at the measurement area, and is synchronous with the changes in transmitted light intensity and reflection-mode cross-polarized light intensity measured independently. The system also provides depth-resolved phase changes as an additional indication of activity. With further investigation our results could open a new era in functional imaging technology to localize neural activity at different depths in situ.