The cytotoxicology of momordicins I and II on Spodoptera litura cultured cell line SL-1
The cytotoxicology of momordicins I and II on Spodoptera litura cultured cell line SL-1
复制标题
苦瓜素 I 和 II 对斜纹夜蛾培养细胞系 SL-1 的细胞毒理学
DOI：
10.1016/j.pestbp.2014.12.007
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发表时间：
2015-07-01
影响因子：
4.7
通讯作者：
Ling, Bing
中科院分区：
文献类型：
--
作者：
Liu, Huan;Wang, Guo-Cai;Ling, Bing
苦瓜甙I和II是苦瓜（Momorphia charantia L.）对斜纹夜蛾卵巢细胞系（SL-1细胞）具有毒性。苦参碱I和II均能显著抑制SL-1细胞增殖。24 h后的IC 50值分别为8.35和82.31 μ g/mL，36 h为6.11和77.49 μ g/mL，48 h为4.93和49.42 μ g/mL。印楝素A对照在24、36和48 h的IC 50值分别为149.63、54.54和23.66 μ g/mL，表明苦参素I的细胞毒性显著高于苦参素II和印楝素A。倒置相差显微镜观察发现，在I和II中，在24 h暴露于苦参碱后，细胞形状变为圆形，肿胀增加，粘附能力下降，细胞膜起泡。结果表明，苦参素Ⅰ对细胞骨架的毒性大于苦参素Ⅱ，苦参素Ⅰ和苦参素Ⅱ处理48 h后，细胞对葡萄糖的吸收抑制率分别为23.04%和13.38%，60 h后分别为47.60%和20.92%。流式细胞仪分析表明，经苦参碱I和II处理的SL-1细胞在细胞周期的G（2）/M期大量积累，48 h后细胞总蛋白含量分别增加了56.93%和35.81%。在I和II中用momorphin处理后，核膜溶解，染色质异常浓缩，核仁受损、迁移或消失。流式细胞仪检测PI荧光值显示，Ⅱ组经苦参素诱导后，SL-1细胞的相对荧光强度分别为521.45和370.17，高于对照组处理48 h后的135.04。这表明细胞膜受到了严重损伤。总体而言，结果表明，抑制细胞骨架功能，有丝分裂图的干扰和核结构的破坏是在I和II暴露的momorphine的影响。这些作用在抑制SL-1细胞生长中起主要作用。苦瓜素抑制昆虫细胞生长和发育的作用模式可用于开发含有葫芦烷型三萜糖苷的新型害虫防治制剂。(C)2014爱思唯尔公司All rights reserved.
Momordicin I and II are secondary metabolites from bitter melon (Momordica charantia L.) that are toxic to the Spodoptera litura ovary cell line (SL-1 cell). Both momordicin I and II significantly inhibited SL-1 cells proliferation. IC50 values after 24 h were 8.35 and 82.31 mu g/mL, 6.11 and 77.49 mu g/mL for 36 h, 4.93 and 49.42 mu g/mL for 48 h for cells treated by momordicin land II, respectively. IC50 values of the azadirachtin A control were 149.63, 54.54 and 23.66 mu g/mL at 24, 36 and 48 h respectively, indicating that the cytotoxicity of momordicin I was significantly higher than that of momordicin II and azadirachtin A. Using inverted phase contrast microscopy we found that after 24 h exposure to momordicin I and II, cell shapes changed to circular, swelling increased, adherence ability declined and the cellular membrane bubbled. After 48 h exposure to momordicin I, most cells were suspended and dead; vacuole deformation and cytoplasm leakage indicated that momordicin I was more toxic to the cytoskeleton than momordicin II.Cells treated with momordicin I and II inhibited glucose absorption by 23.04 and 13.38% after 48 h and 47.60 and 20.92% after 60 h. Flow cytometry analysis suggested that SL-1 cells treated with momordicin I and II dramatically accumulated during the G(2)/M phase of the cell cycle, and total cell protein content increased by 56.93 and 35.81% respectively after 48 h treatment. Following treatment with momordicin I and II the karyotheca dissolved, the chromatin condensed abnormally and the nucleoli were damaged, migrated, or disappeared. The PI fluorescent value by FCM showed that the relative fluorescent intensity of SL-1 cells induced by momordicin land II increased to 521.45 and 370.17, higher than 135.04 induced by control group treatment for 48 h. This indicated significant damage to the cytomembrane. Overall, the results demonstrate that suppression of cytoskeletal function, interference of mitotic figures and destruction of nuclear structure are effects of momordicin I and II exposure. These effects play major roles in momordicin land II inhibition of SL-1 cells growth. The mode of action by which momordicins inhibit insect cell growth and development may be useful in the development of novel pest control formulations containing cucurbitane-type triterpene glycosides. (C) 2014 Elsevier Inc. All rights reserved.